Minor envelope proteins from GP2a to GP4 contribute to the spread pattern and yield of type 2 PRRSV in MARC-145 cells
文献类型： 外文期刊
作者： Bai, Yuan-Zhe 1 ; Sun, Yue 1 ; Liu, Yong-Gang 1 ; Zhang, Hong-Liang 1 ; An, Tong-Qing 1 ; Wang, Qian 1 ; Tian, Zhi-Jun 1 ; Qiao, Xinyuan 2 ; Cai, Xue-Hui 1 ; Tang, Yan-Dong 1 ;
作者机构： 1.Chinese Acad Agr Sci, State Key Lab Anim Dis Control & Prevent, Harbin Vet Res Inst, Harbin, Peoples R China
2.Northeast Agr Univ, Coll Vet Med, Dept Prevent Vet Med, Heilongjiang Key Lab Anim Dis Control & Pharmaceut, Harbin, Peoples R China
3.Heilongjiang Prov Res Ctr Vet Biomed, Harbin Vet Res Inst, Harbin, Peoples R China
4.Harbin Vet Res Inst, Heilongjiang Prov Key Lab Vet Immunol, Harbin, Peoples R China
关键词： spread pattern; PRRSV; cell-to-cell; cell-free; yield
期刊名称：FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY （ 影响因子：5.7； 五年影响因子：5.9 ）
ISSN： 2235-2988
年卷期： 2024 年 14 卷
页码：
收录情况： SCI
摘要： In China, porcine reproductive and respiratory syndrome virus (PRRSV) vaccines are widely used. These vaccines, which contain inactivated and live attenuated vaccines (LAVs), are produced by MARC-145 cells derived from the monkey kidney cell line. However, some PRRSV strains in MARC-145 cells have a low yield. Here, we used two type 2 PRRSV strains (CH-1R and HuN4) to identify the genes responsible for virus yield in MARC-145 cells. Our findings indicate that the two viruses have different spread patterns, which ultimately determine their yield. By replacing the viral envelope genes with a reverse genetics system, we discovered that the minor envelope proteins, from GP2a to GP4, play a crucial role in determining the spread pattern and yield of type 2 PRRSV in MARC-145 cells. The cell-free transmission pattern of type 2 PRRSV appears to be more efficient than the cell-to-cell transmission pattern. Overall, these findings suggest that GP2a to GP4 contributes to the spread pattern and yield of type 2 PRRSV.
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