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Molecular and Biological Characteristics of a Peach Latent Mosaic Viroid PC Isolate in Peach from China: Base Mutations in Hairpin Stems and Implications for Symptomatology

文献类型： 外文期刊

作者： Lu, Meiguang 1 ; Zhang, Zimeng 1 ; Huang, Wen 1 ; Zhou, Jun 2 ; Zhang, Zhixiang 1 ; Li, Shifang 1 ;

作者机构： 1.Chinese Acad Agr Sci, State Key Lab Biol Plant Dis & Insect Pests, Inst Plant Protect, Beijing 100193, Peoples R China

2.Gannan Normal Univ, Natl Navel Orange Engn Res Ctr, Ganzhou 341000, Jiangxi, Peoples R China

关键词： base mutation; hairpin insertion; PC variant; peach latent mosaic viroid; PLMVd infectivity; symptomatology; topping treatment

期刊名称：PLANT DISEASE （ 影响因子：4.4； 五年影响因子：4.8 ）

ISSN： 0191-2917

年卷期： 2024 年 108 卷 7 期

页码：

收录情况： SCI

摘要： Peach latent mosaic viroid (PLMVd) infects peach trees in China and induces a conspicuous albino phenotype (peach calico, PC) that is closely associated with variants containing a 12-to-14 nucleotide hairpin insertion capped by a U-rich loop. Initially, PC disease distribution was limited to parts of Italy, and it was first detected in the field in China in 2019. To explore the molecular and biological characteristics of PLMVd PC isolates in peach in China, we conducted a comprehensive analysis of disease phenotype development and investigated the data-associated pathogenicity and in vivo dynamics of the Chinese isolate PC-A2 using slash-inoculation into GF-305 peach seedlings. Inoculated seedlings displayed PC symptoms much earlier following topping treatment, and PLMVd infectivity was further assessed using bioassay and semiquantitative RT-PCR experiments. Evolutionary analysis showed that the PC isolate and its progeny variants clustered into a single phylogroup distinct from reference PC-C40 isolates from Italy and PC-K1 and PC-K2 from South Korea. Some PC-A2 progeny variants from green leaves of PC-expressing seedlings showed unbalanced point mutations in hairpin stems compared with the PC-C40 reference sequence and constituted a new stem insertion type. The results reveal associations between the recessive phenotypes of peach albino symptoms and base variation in hairpin stem insertions relative to the PC-C40/chloroplastic heat shock protein 90 reference sequence.

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