文献类型： 外文期刊

作者： Liang, Benmeng ¹ ; Bai, Tianyou ¹ ; Zhao, Yuhetian ¹ ; Han, Jiangang ¹ ; He, Xiaohong ¹ ; Pu, Yabin ¹ ; Wang, Chunxin ⁶ ; Liu, Wujun ⁷ ; Ma, Qing ⁸ ; Tian, Kechuan ⁹ ; Zheng, Wenxin ¹⁰ ; Liu, Nan ¹¹ ; Liu, Jianfeng ² ; Ma, Yuehui ¹ ; Jiang, Lin ¹ ;

作者机构： 1.Chinese Acad Agr Sci CAAS, Natl Germplasm Ctr Domest Anim Resources, Inst Anim Sci, Minist Technol, Beijing, Peoples R China

2.China Agr Univ, Coll Anim Sci & Technol, Natl Engn Lab Anim Breeding, Beijing 100193, Peoples R China

3.China Agr Univ, Coll Anim Sci & Technol, MOA Key Lab Anim Genet & Breeding, Beijing 100193, Peoples R China

4.Minist Agr & Rural Affairs, Key Lab Livestock & Poultry Resources Cattle Evalu, Beijing, Peoples R China

5.Univ Coll Dublin, UCD Coll Hlth & Agr Sci, UCD Sch Agr & Food Sci, Anim Genom Lab, Dublin D04V1W8, Ireland

6.Jilin Acad Agr Sci, Inst Anim Sci, Changchun 136100, Peoples R China

7.Xinjiang Agr Univ, Coll Anim Sci, Urumqi, Xinjiang, Peoples R China

8.Ningxia Acad Agr & Forestry Sci, Inst Anim Sci, Yinchuan 75002, Ningxia, Peoples R China

9.Shandong Acad Agr Sci, Inst Anim Sci & Vet Med, Jinan, Peoples R China

10.Xinjiang Acad Anim Sci, Urumqi 830000, Peoples R China

11.Qingdao Agr Univ, Coll Anim Sci & Technol, Qingdao, Peoples R China

关键词： Chinese fine wool sheep; Whole genome sequencing; Genomic selection signature; Follicle density; Missense; Regulatory

期刊名称：JOURNAL OF ADVANCED RESEARCH （ 影响因子：10.7； 五年影响因子：11.4 ）

ISSN： 2090-1232

年卷期： 2024 年 57 卷

页码：

收录情况： SCI

摘要： Introduction: Fine -wool sheep are the most common breed used by the wool industry worldwide. Finewool sheep have over a three -fold higher follicle density and a 50% smaller fiber diameter than coarsewool sheep. Objectives: This study aims to clarify the underlying genetic basis for the denser and finer wool phenotype in fine -wool breeds. Method: Whole-genome sequences of 140 samples, Ovine HD630K SNP array data of 385 samples, including fine, semi -fine, and coarse wool sheep, as well as skin transcriptomes of nine samples were integrated for genomic selection signature analysis. Results: Two loci at keratin 74 ( KRT74 ) and ectodysplasin receptor ( EDAR ) were revealed. Fine -scale analysis in 250 fine/semi-fine and 198 coarse wool sheep narrowed this association to one C/A missense variant of KRT74 (OAR3:133,486,008, P = 1.02E-67) and one T/C SNP in the regulatory region upstream of EDAR (OAR3:61,927,840, P = 2.50E-43). Cellular over -expression and ovine skin section staining assays confirmed that C-KRT74 activated the KRT74 protein and specifically enlarged cell size at the Huxley's layer of the inner root sheath ( P < 0.01). This structure enhancement shapes the growing hair shaft into the finer wool than the wild type. Luciferase assays validated that the C -to -T mutation upregulated EDAR mRNA expression via a newly created SOX2 binding site and potentially led to the formation of more hair placodes. Conclusions: Two functional mutations driving finer and denser wool production were characterized and offered new targets for genetic breeding during wool sheep selection. This study not only provides a theoretical basis for future selection of fine wool sheep breeds but also contributes to improving the value of wool commodities. (c) 2024 The Authors. Published by Elsevier B.V. on behalf of Cairo University. This is an open access article under the CC BY -NC -ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).