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Isolation, identification, and quantification of triterpene saponins in the fresh fruits of Panax notoginseng

文献类型： 外文期刊

作者： Qu, Zheng-yi 1 ; Wang, He-cheng 2 ; Jin, Yin-ping 1 ; Li, Ya-li 1 ; Wang, Ying-ping 3 ;

作者机构： 1.Chinese Acad Agr Sci, Inst Special Wild Econ Anim & Plants, Changchun, Peoples R China

2.Dalian Univ Technol, Sch Life Sci & Med, Panjin, Liaoning, Peoples R China

3.Jilin Agr Univ, State Local Joint Engn Res Ctr Ginseng Breeding &, Changchun, Peoples R China

关键词： Panax notoginseng; fruits; saponins; NMR; quantification

期刊名称：NATURAL PRODUCT RESEARCH （ 影响因子：2.862； 五年影响因子：2.572 ）

ISSN： 1478-6419

年卷期：

页码：

收录情况： SCI

摘要： This study is to develop a method for isolation, identification, and quantitative determination of dammarane-type triterpene saponins in the Panax notoginseng fruits (PNF). The saponins were isolated by a serious of chromatographic methods, and their structures were elucidated on the basis of spectroscopic evidence and comparison with those of literature reports. Quantitative assay was performed on an ultra-performance liquid chromatography-UV (UPLC-UV) method. As a result, 22 saponins were isolated from the extract of PNF, among them, compound 1 was a new saponin, named as malonylgypenoside IX, compounds 3-10, and 14-18 were isolated from the PNF for the first time. As to quantitative analysis, the calibration curves showed good linearity (r > 0.998) within the concentration range, and the method validation provided good reproducibility and sensitivity for the quantification of eight major saponins with precision and accuracy of less than 3.0%.

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