Proteomic analysis of dysregulated pulmonary protein expression and potential pathways in broilers induced by particulate matter exposure in poultry houses
文献类型： 外文期刊
作者： Shen, Dan 1 ; Wang, Kai 1 ; Guo, Zhendong 2 ; Huang, Kai 1 ; Li, Yansen 1 ; Li, Chunmei 1 ;
作者机构： 1.Nanjing Agr Univ, Coll Anim Sci & Technol, Res Ctr Livestock Environm Control & Smart Prod, Nanjing 210095, Peoples R China
2.Chinese Acad Agr Sci, Changchun Vet Res Inst, State Key Lab Pathogen & Biosecur, Key Lab Jilin Prov Zoonosis Prevent & Control, 573 Tulip St, Changchun 130122, Jilin, Peoples R China
关键词： Broiler; Particulate matter; Lung; Proteomics
期刊名称：POULTRY SCIENCE （ 影响因子：4.2； 五年影响因子：4.5 ）
ISSN： 0032-5791
年卷期： 2025 年 104 卷 9 期
页码：
收录情况： SCI
摘要： Airborne particulate matter (PM) generated by intensive broiler production poses significant risks to poultry respiratory health, yet the molecular mechanisms underlying pulmonary injury remain poorly defined. In this study, PM was collected from a commercial broiler house. Fourteen-day-old AA broilers were exposed to fresh air (control, 0.47 mg & sdot;m-3), or 4 and 8 mg & sdot;m-3 total suspended particulates (TSP) for 7 days. iTRAQ-based proteomic profiling identified 4,605 proteins, with 64 differentially expressed (16 upregulated, 48 downregulated) in the high-dose group (8 mg & sdot;m-3). Enrichment analysis revealed involvement of metabolic pathways, phosphatidylinositol signaling, ATP-binding cassette (ABC) transporters, autophagy, and phagosome-related processes. Key hub proteins-PIK3CD, PIK3C2 alpha, SGK3, and MAP3K20-were highlighted by protein-protein interaction networks and validated by ELISA. Their expression levels correlated significantly with cytokine responses, microbial community shifts, and lung metabolites. RT-qPCR confirmed activation of the MyD88-dependent TLR4/NF-kappa B and MAPK signaling cascades, along with oxidative stress and apoptotic markers. These findings uncover a PI3K/ Akt-centered inflammatory pathway in PM-induced broiler lung injury, offering novel targets for improving poultry respiratory health.
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