Inhibition of multiple SARS-CoV-2 variants entry by Lycium barbarum L. polysaccharides through disruption of spike protein-ACE2 interaction
文献类型： 外文期刊
作者： Li, Jingxuan 1 ; Liu, Feng 1 ; Wu, Fushan 1 ; Su, Xiaoyue 1 ; Zhang, Lihui 1 ; Zhao, Xueru 1 ; Shang, Chao 3 ; Han, Lu 1 ; Zhang, Yongxiang 1 ; Xiao, Zhiyong 1 ; Zhou, Wenxia 1 ;
作者机构： 1.Beijing Inst Pharmacol & Toxicol, Beijing 100850, Peoples R China
2.Beijing Inst Pharmacol & Toxicol, State Key Lab Toxicol & Med Countermeasures, Beijing, Peoples R China
3.Chinese Acad Agr Sci Changchun CASS, Changchun Vet Res Inst, Jilin 130122, Peoples R China
关键词： Lycium barbarum L. polysaccharides (LBP); SARS-CoV-2; Pseudovirus; Entry inhibitor; Spike-ACE2 interaction
期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：8.2； 五年影响因子：7.8 ）
ISSN： 0141-8130
年卷期： 2024 年 261 卷
页码：
收录情况： SCI
摘要： Viral respiratory infections are major human health concerns. The most striking epidemic disease, COVID-19 is still on going with the emergence of fast mutations and drug resistance of pathogens. A few polysaccharide macromolecules from traditional Chinese medicine (TCM) have been found to have direct anti-SARS-CoV-2 activity but the mechanism remains unclear. In this study, we evaluated the entry inhibition effect of Lycium barbarum polysaccharides (LBP) in vitro and in vivo. We found LBP effectively suppressed multiple SARS-CoV-2 variants entry and protected K18-hACE2 mice from invasion with Omicron pseudovirus (PsV). Moreover, we found LBP interfered with early entry events during infection in time-of-addition (TOA) assay and SEM observation. Further surface plasmon resonance (SPR) study revealed the dual binding of LBP with Spike protein and ACE2, which resulted in the disruption of Spike-ACE2 interaction and subsequently triggered membrane fusion. Therefore, LBP may act as broad-spectrum inhibitors of virus entry and nasal mucosal protective agent against newly emerging respiratory viruses, especially SARS-CoV-2.
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