An inoculation site-retained mRNA vaccine induces robust immune responses against SARS-CoV-2 variants
文献类型： 外文期刊
作者： Huang, Lei 1 ; Zhao, Fanfan 1 ; He, Muye 1 ; Fang, Yi 1 ; Ma, Xiaoping 1 ; Lu, Shuaiyao 3 ; Li, Entao 2 ; Xiao, Hui 1 ; Zhu, Hanfei 1 ; Wang, Xueli 1 ; Tang, Siyuan 1 ; Yu, Bo 1 ; Wang, Jie 1 ; Zhao, Dong 4 ; Wang, Chao 4 ; Li, Hangwen 1 ; Gao, Yuwei 2 ; Peng, Xiaozhong 3 ; Shen, Haifa 1 ;
作者机构： 1.Stemirna Therapeut, Shanghai 201206, Peoples R China
2.Chinese Acad Agr Sci, Changchun Vet Res Inst, Changchun 130122, Peoples R China
3.Chinese Acad Med Sci & Peking Union Med Coll, Inst Med Biol, Yunnan Key Lab Vaccine Res & Dev Severe Infect Dis, Kunming 650118, Peoples R China
4.Fudan Univ, Dept Mat Sci, Shanghai 200433, Peoples R China
关键词： mRNA vaccine; Inoculation site -retained nanoparticle; COVID-19; SARS-CoV-2
期刊名称：JOURNAL OF CONTROLLED RELEASE （ 影响因子：10.8； 五年影响因子：10.2 ）
ISSN： 0168-3659
年卷期： 2024 年 366 卷
页码：
收录情况： SCI
摘要： mRNA-based vaccines and therapeutic agents hold great promise in prevention and treatment of human diseases, yet high percentage of systemic adverse effect in clinic remains a big safety concern. One major potential cause is a high level of leakage of the locally inoculated mRNA vaccine nanoparticles into circulation. We have screened and optimized a core-shell structured lipopolyplex (LPP) formulation for mRNA with a tissue-retention property. Upon intramuscular inoculation, the mRNA-encapsulated LPP nanoparticles were preferentially taken up by the phagocytic antigen-presentation cells, and potently promoted dendritic cell maturation. We applied the new formulation to prepare a prophylactic vaccine for SARS-CoV-2, and observed potent humoral and cellular immune responses from the vaccine in both murine models and non-human primates. More importantly, the vaccine demonstrated a benign safety profile in non-human primates, with limited side effects after repeated treatment with high dosages of LPP/mRNA. Taken together, the inoculation site-retained vaccine formulation serves as a promising vehicle for mRNA vaccines and therapeutic agents.
- 相关文献
作者其他论文 更多>>
-
Evolutionary dynamics of mitochondrial genomes and intracellular transfers among diploid and allopolyploid cotton species
作者：Kong, Jiali;Wang, Jie;Nie, Liyun;Wu, Zhiqiang;Kong, Jiali;Zou, Changsong;Wu, Zhiqiang;Kong, Jiali;Wang, Jie;Nie, Liyun;Tembrock, Luke R.;Kan, Shenglong;Ma, Xiongfeng;Wendel, Jonathan F.
关键词：Cotton; Mitochondrial genome; Plastid genome; Alternative conformation; Evolutionary rate variation; Intracellular transfer
-
Comprehensive physicochemical indicators analysis and quality evaluation model construction for the post-harvest ripening rapeseeds
作者：Xu, Qiuhui;Wang, Jie;Wang, Dan;Lv, Xin;Fu, Li;He, Ping;Mei, Desheng;Chen, Hong;Wei, Fang;Wei, Fang
关键词：Comprehensive evaluation model; Lipidomics; Physicochemical indicators; Post-harvest ripening; Quality improvement; Rapeseeds
-
Oocytes maintain low ROS levels to support the dormancy of primordial follicles
作者：Qin, Shaogang;Chi, Xinyue;Zhu, Zijian;Gao, Meng;Zhao, Ting;Zhang, Jingwen;Zheng, Wenying;Chen, Ziqi;Zhou, Bo;Xia, Guoliang;Wang, Chao;Chen, Chuanhe;Zhang, Tuo;He, Meina;Zhang, Lifan;Wang, Wenji
关键词：ferroptosis; primary ovarian insufficiency; primordial follicle; ROS; SOD1
-
Multiwalled Carbon Nanotubes Promoted Biofilm Formation and Rhizosphere Colonization of Bacillus subtilis Tpb55
作者：Lv, Xiaolin;Liu, Shanshan;Zhang, Chengsheng;Huang, Bin;Wang, Jie;Cao, Yi;Wu, Huagen
关键词：MWCNTs; biofilm; rhizosphere colonization; Bacillus subtilis; extracellular polymeric substances
-
Chinese yam polysaccharide induces the differentiation and natural antibody secretion of IgM plus B cells to prevent Aeromonas hydrophila infection in grass carp
作者：Tian, Tian-Tian;Wang, Jie;Pan, Yi-Ru;Han, Xue-Qing;Hu, Ya-Zhen;Li, Jie;Zhang, Yong-An;Zhang, Xu-Jie;Zhang, Yong-An;Zhang, Xu-Jie;Zhang, Yong-An;Zhang, Xu-Jie
关键词：Immunostimulant; Chinese yam polysaccharide; Grass carp; IgM plus B cell; IgM
-
CsCIPK20 Improves Tea Plant Cold Tolerance by Modulating Ascorbic Acid Synthesis Through Attenuation of CsCSN5-CsVTC1 Interaction
作者：Di, Taimei;Wu, Yedie;Wang, Jie;He, Mingming;Huang, Jianyan;Li, Nana;Hao, Xinyuan;Ding, Changqing;Zeng, Jianming;Yang, Yajun;Wang, Xinchao;Wang, Lu
关键词：ascorbic acid; CsCIPK20; CsVTC1; low temperature
-
Genome-Wide Silencer Screening Reveals Key Silencer Modulating Reprogramming Efficiency in Mouse Induced Pluripotent Stem Cells
作者：Zhu, Xiusheng;Huang, Lei;Li, Guoli;Deng, Biao;Wang, Xiaoxiao;Yang, Hu;Zhang, Yuanyuan;Wen, Qiuhan;Wang, Chao;Zhang, Jingshu;Li, Kui;Liu, Yuwen;Deng, Biao;Deng, Biao;Zhao, Yunxiang
关键词：
cis -regulatory elements; high-throughput; iPSCs; silencer; Ss-STARR-seq
