Rapid generation of fragrant thermo-sensitive genic male sterile rice with enhanced disease resistance via CRISPR/Cas9
文献类型： 外文期刊
作者： Zhang, Huali 1 ; Liang, Minmin 1 ; Chen, Junyu 1 ; Wang, Huimei 1 ; Ma, Liangyong 1 ;
作者机构： 1.China Natl Rice Res Inst, State Key Lab Rice Biol & Breeding, Hangzhou 311400, Peoples R China
2.China Natl Rice Res Inst, Chinese Natl Ctr Rice Improvement, Hangzhou 311400, Peoples R China
关键词： Bacterial blight resistance; Blast resistance; OsBADH(2); TGMS
期刊名称：PLANTA （ 影响因子：4.3； 五年影响因子：4.8 ）
ISSN： 0032-0935
年卷期： 2024 年 259 卷 5 期
页码：
收录情况： SCI
摘要： Main conclusionThe three, by mutagenesis produced genes OsPi21, OsXa5, and OsBADH2, generated novel lines exhibiting desired fragrance and improved resistance.AbstractElite sterile lines are the basis for hybrid rice breeding, and rice quality and disease resistance become the focus of new sterile lines breeding. Since there are few sterile lines with fragrance and high resistance to blast and bacterial blight at the same time in hybrid rice production, we here integrated the simultaneous mutagenesis of three genes, OsPi21, OsXa5, and OsBADH2, into Zhi 5012S, an elite thermo-sensitive genic male sterile (TGMS) variety, using the CRISPR/Cas9 system, thus eventually generated novel sterile lines would exhibit desired popcorn-like fragrance and improved resistance to blast and bacterial blight but without a loss in major agricultural traits such as yield. Collectively, this study develops valuable germplasm resources for the development of two-line hybrid rice with disease resistance, which provides a way to rapid generation of novel TGMS lines with elite traits.
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