文献类型： 外文期刊

作者： Tang, Liqun ¹ ; Li, Guanghao ¹ ; Wang, Huimei ¹ ; Zhao, Juan ¹ ; Li, Zhiyong ¹ ; Liu, Xixi ¹ ; Shu, Yazhou ¹ ; Liu, Wanning ¹ ; Wang, Shuang ¹ ; Huang, Jie ¹ ; Ying, Jiezheng ¹ ; Tong, Xiaohong ¹ ; Yuan, Wenya ⁴ ; Wei, Xiangjin ¹ ; Tang, Shaoqing ¹ ; Wang, Yifeng ¹ ; Bu, Qingyun ² ; Zhang, Jian ¹ ;

作者机构： 1.China Natl Rice Res Inst, State Key Lab Rice Biol & Breeding, Hangzhou 311400, Peoples R China

2.Chinese Acad Sci, Northeast Inst Geog & Agroecol, Key Lab Soybean Mol Design Breeding, Harbin 150081, Peoples R China

3.Chinese Acad Sci, Innovat Acad Seed Design, Beijing 100101, Peoples R China

4.Hubei Univ, Coll Life Sci, Hubei Collaborat Innovat Ctr Green Transformat Bio, Hubei Key Lab Ind Biotechnol, Wuhan 430062, Peoples R China

关键词： Abscisic acid; bZIP; Flowering; Polycomb Repressive Complex 2 (PRC2); Rice(Oryza sativa L.); SnRK2

期刊名称：JOURNAL OF ADVANCED RESEARCH （ 影响因子：10.7； 五年影响因子：11.4 ）

ISSN： 2090-1232

年卷期： 2024 年 59 卷

页码：

收录情况： SCI

摘要： Introduction: Rice flowering is a major agronomic trait, determining yield and ecological adaptability in particular regions. ABA plays an essential role in rice flowering, but the underlying molecular mechanism remains largely elusive. Objectives: In this study, we demonstrated a "SAPK8-ABF1-Ehd1/Ehd2" pathway, through which exogenous ABA represses rice flowering in a photoperiod-independent manner. Methods: We generated abf1 and sapk8 mutants using the CRISPR-Cas9 method. Using yeast two-hybrid, Pull down, BiFC and kinase assays, SAPK8 interacted and phosphorylated ABF1. ABF1 directly bound to the promoters of Ehd1 and Ehd2 using ChIP-qPCR, EMSA, and LUC transient transcriptional activity assay, and suppressed the transcription of these genes. Results: Under both long day and short day conditions, simultaneous knock-out of ABF1 and its homolog bZIP40 accelerated flowering, while SAPK8 and ABF1 over-expression lines exhibited delayed flowering and hypersensitivity to ABA-mediated flowering repression. After perceiving the ABA signal, SAPK8 physically binds to and phosphorylates ABF1 to enhance its binding to the promoters of master positive flowering regulators Ehd1 and Ehd2. Upon interacting with FIE2, ABF1 recruited PRC2 complex to deposit H3K27me3 suppressive histone modification on Ehd1 and Ehd2 to suppress these genes transcription, thereby leading flowering. Conclusion: Our work highlighted the biological functions of SAPK8 and ABF1 in ABA signaling, flowering control and the involvement of a PRC2-mediated epigenetic repression mechanism in the transcription regulation governed by ABF1 on ABA-mediated rice flowering repression. (c) 2024 The Authors. Published by Elsevier B.V. on behalf of Cairo University. This is an open access article under the CC BY -NC -ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).