文献类型： 外文期刊

作者： Xu, Liping ¹ ; Hao, Jiaxin ¹ ; Lv, Mengfan ¹ ; Liu, Peipei ¹ ; Ge, Qidong ¹ ; Zhang, Sainan ¹ ; Yang, Jianping ¹ ; Niu, Hongbin ¹ ; Wang, Yiru ³ ; Xue, Yadong ¹ ; Lu, Xiaoduo ⁴ ; Tang, Jihua ¹ ; Zheng, Jun ³ ; Gou, Mingyue ¹ ;

作者机构： 1.Henan Agr Univ, Collaborat Innovat Ctr Henan Grain Crops, State Key Lab Wheat & Maize Crops Sci, Zhengzhou 450002, Peoples R China

2.Shennong Lab, Zhengzhou 450002, Peoples R China

3.Chinese Acad Agr Sci, Inst Crop Sci, State Key Lab Crop Gene Resources & Breeding, Beijing 100081, Peoples R China

4.Qilu Normal Univ, Inst Adv Agr Technol, Jinan 250200, Peoples R China

期刊名称：PLANT PHYSIOLOGY （ 影响因子：7.4； 五年影响因子：8.7 ）

ISSN： 0032-0889

年卷期： 2024 年 194 卷 4 期

页码：

收录情况： SCI

摘要： The plant cuticle is essential in plant defense against biotic and abiotic stresses. To systematically elucidate the genetic architecture of maize (Zea mays L.) cuticular wax metabolism, 2 cuticular wax-related traits, the chlorophyll extraction rate (CER) and water loss rate (WLR) of 389 maize inbred lines, were investigated and a genome-wide association study (GWAS) was performed using 1.25 million single nucleotide polymorphisms (SNPs). In total, 57 nonredundant quantitative trait loci (QTL) explaining 5.57% to 15.07% of the phenotypic variation for each QTL were identified. These QTLs contained 183 genes, among which 21 strong candidates were identified based on functional annotations and previous publications. Remarkably, 3 candidate genes that express differentially during cuticle development encode beta-ketoacyl-CoA synthase (KCS). While ZmKCS19 was known to be involved in cuticle wax metabolism, ZmKCS12 and ZmKCS3 functions were not reported. The association between ZmKCS12 and WLR was confirmed by resequencing 106 inbred lines, and the variation of WLR was significant between different haplotypes of ZmKCS12. In this study, the loss-of-function mutant of ZmKCS12 exhibited wrinkled leaf morphology, altered wax crystal morphology, and decreased C32 wax monomer levels, causing an increased WLR and sensitivity to drought. These results confirm that ZmKCS12 plays a vital role in maize C32 wax monomer synthesis and is critical for drought tolerance. In sum, through GWAS of 2 cuticular wax-associated traits, this study reveals comprehensively the genetic architecture in maize cuticular wax metabolism and provides a valuable reference for the genetic improvement of stress tolerance in maize. Genome-wide association study of 2 cuticular wax-associated traits in maize reveals a beta-ketoacyl-CoA synthase gene critical for drought tolerance.