The rescue and selection of thermally stable type O vaccine candidate strains of foot-and-mouth disease virus
文献类型： 外文期刊
作者： Gao, Ya 1 ; Li, Pinghua 1 ; Ma, Xueqing 1 ; Bai, Xingwen 1 ; Sun, Pu 1 ; Du, Ping 1 ; Yuan, Hong 1 ; Cao, Yimei 1 ; Li, Kun; 1 ;
作者机构： 1.Chinese Acad Agr Sci, Lanzhou Vet Res Inst, State Key Lab Vet Etiol Biol, Lanzhou 730046, Gansu, Peoples R China
期刊名称：ARCHIVES OF VIROLOGY （ 影响因子：2.243； 五年影响因子：2.176 ）
ISSN： 0304-8608
年卷期：
页码：
收录情况： SCI
摘要： Inactivated foot-and-mouth disease virus (FMDV) vaccines have been used widely to control foot-and-mouth disease (FMD). However, the virions (146S) of this virus are easily dissociated into pentamer subunits (12S), which limits the immune protective efficacy of inactivated vaccines when the temperature is higher than 30 degrees C. A cold-chain system can maintain the quality of the vaccines, but such systems are usually not reliable in limited-resource settings. Thus, it is imperative to improve the thermostability of vaccine strains to guarantee the quality of the vaccines. In this study, four recombinant FMDV strains containing single or multiple amino acid substitutions in the structural proteins were rescued using a previously constructed FMDV type O full-length infectious clone (pO/DY-VP1). We found that single or multiple amino acid substitutions in the structural proteins affected viral replication to different degrees. Furthermore, the heat and acid stability of the recombinant viruses was significantly increased when compared with the parental virus. Three thermally stable recombinant viruses (rHN/DY-VP1(Y2098F), rHN/DY-VP1(V2090A-S2093H), and rHN/DY-VP1(V2090A-S2093H-Y2098F)) were prepared as inactivated vaccines to immunize pigs. Blood samples were collected every week to prepare sera, and a virus neutralization test showed that the substitutions S2093H and Y2098F, separately or in combination, did not affect the immunogenicity of the virus, but the Y2098F mutation increased the thermostability significantly (p < 0.05). Therefore, the rHN/DY-VP1(Y2098F) mutant should be considered for use in future vaccines.
- 相关文献
作者其他论文 更多>>
-
Development of Genome-Wide SSR Markers in Leymus chinensis with Genetic Diversity Analysis and DNA Fingerprints
作者：Ou, Taiyou;Wu, Zinian;Tian, Chunyu;Yang, Yanting;Gong, Wenlong;Niu, Jianjiang;Li, Zhiyong;Ou, Taiyou;Wu, Zinian;Tian, Chunyu;Yang, Yanting;Gong, Wenlong;Niu, Jianjiang;Li, Zhiyong
关键词：DNA fingerprint; genetic diversity;
Leymus chinensis ; simple sequence repeat; whole-genome sequencing -
Rumen-Degradable Starch Improves Rumen Fermentation, Function, and Growth Performance by Altering Bacteria and Its Metabolome in Sheep Fed Alfalfa Hay or Silage
作者：Guo, Wenliang;Na, Meila;Liu, Shuwei;Du, Haidong;Zhang, Jing;Na, Renhua;Li, Kenan
关键词：alfalfa; rumen-degradable starch; rumen fermentation; growth performance; urea transporter; bacteria and metabolome; microbial protein synthesis
-
Establishment of a Direct Competitive ELISA for Camel FGF21 Detection
作者：Yang, Yuxuan;Zhao, Shuqin;Gao, Yuan;Yang, Yuxuan;Yuan, Hong;Jiao, Yunjuan;Fu, Yuanfang;Bai, Xingwen;Lu, Zengjun
关键词：camel; FGF21; prokaryotic expression; affinity chromatography purification; polyclonal antibody; direct competition ELISA
-
Complete mitochondrial genome of Medicago sativa ssp. falcata (Papilionoideae, Fabaceae): characterization and phylogenetic analysis
作者：Ou, Taiyou;Wu, Zinian;Liu, Qian;Tian, Chunyu;Yang, Yanting;Liu, Lemeng;Guo, Maowei;Li, Zhiyong;Ou, Taiyou;Wu, Zinian;Liu, Qian;Tian, Chunyu;Yang, Yanting;Liu, Lemeng;Guo, Maowei;Li, Zhiyong
关键词：
Medicago falcate ; Mitochondrial genome; Gene transfer; RNA editing; Phylogenetic analysis -
Functional analysis of BmTsp.C in modulating infection of BmNPV through apoptosis pathways in domestic silkworm (Bombyx mori)
作者：Xu, Yuanyuan;Wei, Yimeng;Zhang, Jing;Zhang, Dan;Zhao, Qiaoling;Shen, Dongxu;Xu, Yuanyuan;Wei, Yimeng;Zhang, Jing;Zhang, Dan;Zhao, Qiaoling;Shen, Dongxu
关键词：apoptosis; BmNPV; bombyx mori; tetraspanins
-
Identifications of Genes Involved in ABA and MAPK Signaling Pathways Positively Regulating Cold Tolerance in Rice
作者：Ding, Guohua;Li, Zhugang;Cao, Liangzi;Zhou, Jinsong;Lei, Lei;Luo, Yu;Bai, Liangming;Yang, Guang;Sun, Shichen;Ding, Guohua;Li, Zhugang;Cao, Liangzi;Zhou, Jinsong;Lei, Lei;Luo, Yu;Bai, Liangming;Wang, Rongsheng;Li, Kun;Wang, Xueyang;Liu, Kai;Sun, Shichen;Iqbal, Zubair;Qu, Mingnan;Zhao, Minghui;Cui, Zhibo;Qu, Mingnan
关键词：cold stress; ABA signaling; MAPK signaling; transcriptional regulation; integrative analysis
-
MARCH8 promotes the proteasomal degradation of foot-and-mouth disease virus VP1, VP2, and VP3 to negatively regulate viral replication
作者：Yin, Mengge;Li, Xiangmin;Zhang, Min;Zhao, Qiongqiong;Wang, Haoyuan;Qian, Ping;Yin, Mengge;Li, Xiangmin;Zhang, Min;Zhao, Qiongqiong;Wang, Haoyuan;Qian, Ping;Li, Xiangmin;Qian, Ping;Li, Xiangmin;Qian, Ping;Li, Xiangmin;Qian, Ping;Zhang, Huiyan;Lu, Zengjun
关键词：Membrane-associated RING-CH8 protein; foot-and-mouth disease virus; restriction factor; proteasomal degradation; K33-linked polyubiquitination
