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On-line identification of silkworm pupae gender by short-wavelength near infrared spectroscopy and pattern recognition technology

文献类型： 外文期刊

作者： Ma, Yue 1 ; Xu, Yichao 1 ; Yan, Hui 1 ; Zhang, Guozheng 1 ;

作者机构： 1.Jiangsu Univ Sci & Technol, Sch Biotechnol, Zhenjiang 212018, Jiangsu, Peoples R China

2.Chinese Acad Agr Sci, Sericultural Res Inst, Zhenjiang, Jiangsu, Peoples R China

关键词： Silkworm pupae; gender identification; short-wave near infrared spectroscopy; pattern recognition; partial least squares discriminant analysis

期刊名称：JOURNAL OF NEAR INFRARED SPECTROSCOPY （ 影响因子：1.198； 五年影响因子：1.377 ）

ISSN： 0967-0335

年卷期：

页码：

收录情况： SCI

摘要： The gender identification of silkworm pupae is a critical step in the sericulture industry's breeding process. In this study, a low cost, short-wavelength (815-1075 nm) near infrared (NIR) spectrometer combined with multivariate spectra evaluation methods was used to establish calibration models for the on-line identification of female and male pupae of eight silkworm varieties (Hibiscus, Jingsong, 932, Xiang Hui, 7532xXiang Hui, Haoyue B, Jingsong B, and 7532). The diffuse reflection short-wavelength spectra were recorded, and then principal component analysis (PCA), linear discriminant analysis (LDA), and partial least squares discriminant analysis (PLSDA) were tested for calibration model development. The PCA and LDA results showed, that spectral differences between the female and male silkworm pupae existed, however, the two evaluation techniques could not separate the female and male silkworm pupae with the required accuracy. The PLSDA calibration models, on the other hand, could separate the pupae according to their gender with the necessary prediction accuracy of >98.44%. Thus, it has been proved, that a low-cost, short-wavelength range NIR spectrometer in combination with a PLSDA calibration routine can be successfully applied for the reliable on-line identification of female and male silkworm pupae.

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