您好，欢迎访问中国农业科学院 机构知识库！

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Genome-wide identification and functional prediction of circular RNAs in response to heat stress in Chinese Holstein cows

文献类型： 外文期刊

作者： Qi, Ying 1 ; Zhang, Lin 1 ; Guo, Yuemei 1 ; Wang, Jing 1 ; Chu, Mingxing 2 ; Zhang, Yiming 1 ; Guo, Junfei 3 ; Li, Qiulin 1 ;

作者机构： 1.Langfang Normal Univ, Coll Life Sci, Hebei Key Lab Anim Divers, Langfang Key Lab Cell Engn & Applicat, Aiminxi Rd, Langfang 065000, Peoples R China

2.Chinese Acad Agr Sci, Inst Anim Sci, Key Lab Anim Poultry Genet Breeding & Reprod, Minist Agr & Rural Affairs, Beijing, Peoples R China

3.Guangdong Women & Children Hosp, Clin Lab Dept, 521 Xingnan Rd, Guangzhou 510000, Peoples R China

关键词： Heat stress; circRNAs; mammary gland; noncoding RNA; dairy cows

期刊名称：ANIMAL BIOTECHNOLOGY （ 影响因子：2.282； 五年影响因子：1.702 ）

ISSN： 1049-5398

年卷期：

页码：

收录情况： SCI

摘要： Heat stress (HS) leads to substantial economic loss of dairy industry each year. The negative effect of HS in dairy cows is becoming one of the more urgent issue due to accelerating side-effects of global warming. Various genes are involved in HS response but the information about the role of noncoding RNAs, especially circular RNAs (circRNAs) is largely unknown. In our study, we aimed to investigate the different expression profile of circRNAs between HS and Non-heat-stressed condition (NC) of Chinese Holstein cow's mammary gland. CircRNAs were identified using RNA sequencing and bioinformatics analysis. In total, 37405 circRNAs were detected and 95 were differentially expressed (DE), including 15 downregulated and 80 upregulated circRNAs in HS group compared to NC. Eight circRNAs were randomly selected to verify the RNA sequencing result. Further, Sanger sequencing validated the backsplicing site of the eight circRNAs. Moreover, results obtained from the Quantitative real time PCR (qRT-PCR) showed consistent expression trend with that of RNA sequencing. GO annotation and KEGG analysis suggested that these DE circRNAs probably involved in the energy metabolic regulation. Furthermore, we constructed ceRNA network and the result indicated that these DE circRNAs could regulate lactation through IGF1 and PRL signaling pathway.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台