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Development and Validation of an Analytical Method for the Quantification of Arabinose, Galactose, Glucose, Sucrose, Fructose, and Maltose in Fruits, Vegetables, and Their Products

文献类型： 外文期刊

作者： Wang, Bingfeng 1 ; Wang, Xue 1 ; Bei, Jun 5 ; Xu, Lei 1 ; Zhang, Xinlian 1 ; Xu, Zhenzhen 1 ;

作者机构： 1.Chinese Acad Agr Sci, Inst Qual Stand & Testing Technol Agroprod, Beijing 100081, Peoples R China

2.China Agr Univ, Coll Food Sci & Nutr Engn, Beijing 100083, Peoples R China

3.Natl Res Ctr Fruit & Vegetable Proc Engn, Beijing 100083, Peoples R China

4.Minist Agr & Rural Affairs, Key Lab Fruit & Vegetable Proc, Beijing 100083, Peoples R China

5.Chinese Acad Inspect & Quarantine, Beijing, Peoples R China

关键词： Ion chromatography; Arabinose; Galactose; Glucose; Sucrose; Fructose

期刊名称：FOOD ANALYTICAL METHODS （ 影响因子：3.366； 五年影响因子：3.07 ）

ISSN： 1936-9751

年卷期：

页码：

收录情况： SCI

摘要： The paper presents a method for the simultaneous determination of arabinose, galactose, glucose, sucrose, fructose, and maltose in fruits, vegetables, and their products using ion chromatography-pulsed amperometric detection on a gold working electrode. Sample preparation involved simple extraction, dilution with water, and filtration before injection into the system. Separation of the analytes was performed with gradient elution on a Thermo Scientific CarboPac PA20 Analytical Column (3.0 x 150 mm) and a Guard column (2 mm x 50 mm) with a mobile phase. The optimized parameters were an eluent concentration of 10 mmol/L, a column temperature of 30 degrees C, and a flow rate of 0.4 mL/min. In methodological evaluation, parameters (selectivity, linearity, limit of detection (LOD), limit of quantification (LOQ), relative standard deviation (RSD), recovery percentages, and CV%) resulted in compliance with reference values at each level of fortification. All validation parameters were acceptable in the characterization of sugars in fruits, vegetables, and their products. After method validation, we analyzed the sugar content in fresh and dried jujube. The method was accurate, reliable, and reproducible and can be used for the determination of arabinose, galactose, glucose, sucrose, fructose and maltose in fruits, vegetables, and their products.

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