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Puerarin induces apoptosis in prostate cancer cells via inactivation of the Keap1/Nrf2/ARE signaling pathway

文献类型: 外文期刊

作者: Li, Jianjun 1 ; Xiong, Chuan 3 ; Xu, Pan 4 ; Luo, Qiang 4 ; Zhang, Ronggui 1 ;

作者机构: 1.Chongqing Med Univ, Urol Surg Dept, Affiliated Hosp 2, Chongqing, Peoples R China

2.Tradit Chinese Med Hosp Fengjie, Urol Surg Dept, Chongqing, Peoples R China

3.Sichuan Acad Agr Sci, Biotechnol & Nucl Technol Res Inst, Chengdu, Peoples R China

4.Chongqing Med Univ, Affiliated Hosp 2, Inst Viral Hepatitis, Key Lab Mol Biol Infect Dis,Minist Educ, Chongqing, Peoples R China

关键词: Puerarin; prostate cancer cells; androgen-independent; Apoptosis; Keap1/Nrf2/ARE signaling pathway

期刊名称:BIOENGINEERED ( 影响因子:3.269; 五年影响因子:2.916 )

ISSN: 2165-5979

年卷期: 2021 年 12 卷 1 期

页码:

收录情况: SCI

摘要: In this study, we examined the antitumor effects of Puerarin (PEU) on androgen-independent (DU145 and PC-3) and androgen-dependent (LNCaP) prostate cancer cells, and explored its potential mechanisms. Supplement with PEU (2.5 mu M, 5 mu M, and 10 mu M) exhibited a marked inhibitory effect against the growth of DU145 and PC-3 cells, especially beyond 24 h, whereas there is only slight growth inhibitory effect on LNCaP cells at the high concentration of 10 mu M at 72 h. This loss of cell viability in DU145 and PC-3 cells by PEU was mediated by the induction of apoptosis via up-regulation of Bax and cleaved-caspase-3, but downregulation of Bcl-2. Moreover, more intracellular ROS and LDH production were observed in DU145 and PC-3 cells upon PEU treatment. Meanwhile, the amount of pro-inflammatory cytokines (IL-1 beta and IL-6) was increased, but the content of anti-inflammatory cytokines IL-10 was attenuated. Additionally, PEU pretreatment resulted in an increase of Keap1 protein expression, and a decline of Nrf2, HO-1 and NQO1 protein expression in DU145 and PC3 cells. The present findings indicated that PEU exerted its antitumor activities toward androgen-independent prostate cancer cells via inactivation of Keap1/NrF2/ARE signaling pathway. [GRAPHICS] .

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