Differences in Bacterial Communities of Retail Raw Pork in Different Market Types in Hangzhou, China
文献类型: 外文期刊
作者: Wang, Wen 1 ; Yi, Zhengkai 1 ; Cai, Wei 2 ; Ma, Jiele 1 ; Yang, Hua 1 ; Zhou, Min 2 ; Xiao, Xingning 1 ;
作者机构: 1.Zhejiang Acad Agr Sci, Inst Agroprod Safety & Nutr, State Key Lab Managing Biot & Chem Threats Qual &, MOA Lab Qual & Safety Risk Assessment Agroprod Han, Hangzhou 310021, Peoples R China
2.Wuhan Polytech Univ, Coll Food Sci & Engn, Wuhan 430048, Peoples R China
关键词: pork; retail markets; high-throughput sequencing; bacterial community; bacterial contamination
期刊名称:FOODS ( 影响因子:5.2; 五年影响因子:5.5 )
ISSN:
年卷期: 2023 年 12 卷 18 期
页码:
收录情况: SCI
摘要: Pork is widely consumed globally, and pigs' microbiota can potentially harbor foodborne pathogens. Contaminated pork in retail markets poses significant implications for food quality and safety. However, limited studies have compared pork microbiomes in various marketing environments. In this study, we utilized traditional microbial culture methods and high-throughput 16S rRNA sequencing to assess pathogen contamination and bacterial diversity in raw pork samples purchased from farmers' markets and two types of supermarkets (upscale and ordinary) in Hangzhou, China. Traditional microbial plate cultures identified E. coli and Salmonella spp. in 32.1% (27/84) and 15.5% (13/84) of the collected pork samples, respectively. Moreover, 12 out of 13 Salmonella strains were found in farmers' markets. The MIC results indicated a high prevalence of MDR strains, accounting for 51.9% in E. coli and 53.8% in Salmonella. The prevalence of NaClO tolerant strains was 33.3% and 92.3% for E. coli and Salmonella, respectively. Sequencing results indicated significantly higher microbial diversity in farmers' market samples compared to supermarket samples. Farmers' market pork samples exhibited a greater abundance of Acinetobacter, while Pseudomonas and Brochothrix were predominant in supermarket samples. The total abundance of pathogenic and spoilage bacteria was also higher for the farmers' market samples. Cross-contamination during market trading was evident through a high correlation between bacterial abundance in pork from different stalls within the same farmers' market. PICRUSt2 analysis identified significant differences in the average proportions of genes for carbohydrate, energy, and lipid metabolism from the farmers' markets, suggesting an exacerbation of microbial metabolic activity and increased perishability of pork in this environment. In conclusion, this study revealed variations in the characteristics of raw pork bacterial contamination across different types of retail stores, as well as differences in the composition and diversity of their respective bacterial communities.
- 相关文献
作者其他论文 更多>>
-
Quantitative characterization and dynamics of bacterial communities in ready-to-eat chicken using high-throughput sequencing combined with internal standard-based absolute quantification
作者:Yi, Zhengkai;Qiu, Mengjia;Xiao, Xingning;Ma, Jiele;Yang, Hua;Wang, Wen;Qiu, Mengjia
关键词:High-throughput sequencing; Internal standard method; Ready-to-eat chicken; Absolute abundance
-
Combining nanoflares biosensor and mathematical resolution technique for multi-class mycotoxin analysis in complex food matrices
作者:Ji, Xiaofeng;Xiao, Yingping;Wang, Wen;Lyu, Wentao;Yang, Hua;Liu, Zhi;Xi, Yang;Wang, Tong;Qi, Zuo
关键词:Nanoflares; Mycotoxins; Multispectral resolution; Second -order calibration; Food safety control
-
Evaluation of Alternaria toxins in fruits, vegetables and their derivatives marketed in China using a QuEChERS method coupled with ultra-high performance liquid chromatography-tandem mass spectrometry: Analytical methods and occurrence
作者:Ji, Xiaofeng;Xiao, Yingping;Lyu, Wentao;Wang, Wen;Tang, Biao;Yang, Hua;Deng, Tao;Wu, Zhen;Jin, Canghong;Yang, Hua
关键词:Mycotoxins; Alternaria; Food safety; QuEChERS
-
Aminobenzaldehyde convelently modified graphitic carbon nitride photocatalyst through Schiff base reaction: Regulating electronic structure and improving visible-light-driven photocatalytic activity for moxifloxacin degradation
作者:Liu, Yi;Bian, Changhao;Li, Yingshuang;Sun, Pengfei;Dong, Xiaoping;Xiao, Yingping;Xiao, Xingning;Wang, Wen
关键词:Hybrid graphitic carbon nitride; Schiff base reaction; Aminobenzaldehyde; Moxifloxacin; Photocatalytic mechanism
-
Emerging Alternaria and Fusarium mycotoxins in tomatoes and derived tomato products from the China market: Occurrence, methods of determination, and risk evaluation
作者:Ji, Xiaofeng;Xiao, Yingping;Lyu, Wentao;Wang, Wen;Wang, Xiaoli;Yang, Hua;Deng, Tao;Wu, Zhen;Jin, Canghong;He, Qinfang;Yang, Hua
关键词:Emerging; Alternaria; Fusarium; Mycotoxin; Tomato; Dietary exposure
-
The Dissipation Behavior and Risk Assessment of Carbendazim Under Individual and Joint Applications on Peach (Amygdalus persica L.)
作者:Qian, Mingrong;Zhou, Min;Li, Yue;Wu, Huizhen;Wang, Dou;Yang, Guiling;Yao, Liping
关键词:Carbendazim; Dissipation; Joint application; Peach; Risk assessment
-
Engineering Escherichia coli Nissle 1917 as a microbial chassis for therapeutic and industrial applications
作者:Yu, Mingjing;Hu, Shilong;Sun, Dongchang;Tang, Biao;Yang, Hua
关键词:Escherichia coli Nissle 1917; Living therapeutics; CRISPR-Cas; Light-controlled gene expression; 'Suicide' systems; Minicells; Bacterial ghosts