文献类型： 外文期刊

作者： Chai, Changpeng ¹ ; Tang, Huan ² ; Yi, Jianfeng ³ ; Li, Lu ¹ ; Yu, Cheng ² ; Su, Yuanhui ² ; Miao, Long ¹ ; Ye, Zhenzhen ² ; Wang, Zhengfeng ¹ ; Luo, Wei ¹ ; Hu, Jinjing ¹ ; Zhang, Hui ² ; Miao, Xin ⁸ ; Xu, Hao ¹ ; Zhou, Wence ² ;

作者机构： 1.Lanzhou Univ, Hosp 1, Dept Gen Surg 4, Lanzhou 730000, Peoples R China

2.Lanzhou Univ, Clin Med Coll 2, Lanzhou 730000, Peoples R China

3.Lanzhou Univ, Clin Med Coll 1, Lanzhou 730000, Peoples R China

4.Gansu Univ Chinese Med, Sch Clin Med 1, Dept Surg, Lanzhou 730000, Peoples R China

5.Lanzhou Univ, Hosp 2, Dept Anesthesiol, Lanzhou 730000, Peoples R China

6.Gansu Univ Chinese Med, Sch Clin Med 1, Lanzhou, Gansu, Peoples R China

7.Lanzhou Univ, Hosp 2, Dept Gen Surg, 82 Cuiyingmen, Lanzhou 730000, Peoples R China

8.Chinese Acad Agr Sci, Lanzhou Vet Res Inst, State Key Lab Vet Etiol Biol, Key Lab Anim Virol,Minist Agr, Lanzhou 730046, Peoples R China

关键词： Mixed-type ampullary cancer; DPC-X4 cell line; Drug sensitivity; Xenograft tumor model; Pathogenetic research

期刊名称：HUMAN CELL （ 影响因子：4.3； 五年影响因子：4.2 ）

ISSN： 0914-7470

年卷期： 2024 年 37 卷 2 期

页码：

收录情况： SCI

摘要： Mixed-type ampullary cancer is a distinct subtype of ampullary cancer that manifests a merging of the biological characteristics of both intestinal and pancreaticobiliary subtypes. The absence of established cell lines specific to this subtype has resulted in a concomitant scarcity of research on its tumorigenic mechanisms and the development of novel therapeutic modalities. The present study achieved the successful establishment of a novel mixed-type ampullary cancer cell line, designated DPC-X4 through primary culture techniques. Subsequent analyses pertaining to phenotypic characteristics, molecular profiling, biomarker identification, and histological features validated the DPC-X4 cell line as a potent model for delineating the pathogenesis of mixed-type ampullary cancer and facilitating the development of new pharmacological agents. This newly established cell line was subjected to continuous cultivation for 1 year, with stable passaging for over 50 generations. Notably, the DPC-X4 cell line manifested typical morphological features associated with epithelial tumors. Furthermore, the population doubling time for the DPC-X4 cell line was determined at 70 h. Short tandem repeat (STR) analysis confirmed that the DPC-X4 cell line exhibited a high genetic concordance with the primary tumor from the patient. Karyotypic profiling indicated an abnormal sub-triploid karyotype, with representative karyotypes of 57, XXY inv (9), 14p + , 15p + , der (17), + mar. The DPC-X4 cell line demonstrated a high capacity for efficient organoid formation under suspension culture conditions. In addition, the subcutaneous inoculation of DPC-X4 cells into NXG mice led to the formation of xenografted tumors. The results of drug sensitivity testing indicated that DPC-X4 cells were sensitive to paclitaxel and resistant to oxaliplatin, 5-fluorouracil, and gemcitabine. Immunohistochemistry revealed positive expression of CK7, CK19, and CK20 in DPC-X4 cells, while CDX2 demonstrated negative expression. In addition, positive expression of E-cadherin and vimentin was identified in DPC-X4 cells, with a proliferation index indicated by Ki-67 at 70%. The findings of our study establish DPC-X4 as a novel mixed-type ampullary cancer cell line, which can serve as a potential experimental model for exploring the pathogenesis of ampullary cancer and the development of therapeutic drugs.