Auxin Biosynthesis Is Required for Phosphorus Deficiency-Induced Root Architecture Remodelling in Rice
文献类型： 外文期刊
作者： Kang, Shujing 1 ; Li, Zeyu 1 ; Zhang, Guangzhong 1 ; Zhang, Yuxin 1 ; Wang, Yuexing 4 ; Wang, Quan 1 ; Wang, Suikang 1 ;
作者机构： 1.Chinese Acad Agr Sci, Agr Genom Inst Shenzhen, Guangdong Lab Lingnan Modern Agr, Shenzhen Branch,Genome Anal Lab,Minist Agr & Rural, Shenzhen, Peoples R China
2.Huazhong Agr Univ, Natl Key Lab Crop Genet Improvement, Wuhan, Peoples R China
3.Huazhong Agr Univ, Natl Ctr Plant Gene Res, Wuhan, Peoples R China
4.China Natl Rice Res Inst, State Key Lab Rice Biol, Hangzhou, Peoples R China
5.Nankai Univ, Coll Life Sci, Tianjin, Peoples R China
关键词： auxin biosynthesis; phosphorus starvation; root architecture
期刊名称：PHYSIOLOGIA PLANTARUM （ 影响因子：3.6； 五年影响因子：5.4 ）
ISSN： 0031-9317
年卷期： 2025 年 177 卷 3 期
页码：
收录情况： SCI
摘要： Phosphorus (P) deficiency severely restricts plant growth due to its low mobility in soil, driving the evolution of adaptive root system architecture (RSA) remodelling. While auxin has been implicated in P deficiency responses, the molecular mechanisms governing RSA plasticity in rice (Oryza sativa) remain unclear. Here, we identify LRLP1/OsTAR2 and OsYUC8 as key regulators of P-dependent RSA modifications through auxin biosynthesis. The lrlp1 mutant, exhibiting impaired lateral root (LR) and root hair (RH) development under low P, harbors a loss-of-function mutation in OsTAR2, a tryptophan aminotransferase essential for indole-3-pyruvic acid (IPyA)-dependent auxin production. Similarly, CRISPR-Cas9 knockout of OsYUC8, a downstream flavin monooxygenase in the auxin pathway, attenuated LR and RH responses to P stress. Spatial analysis using DR5::VENUS revealed P-deficiency-enhanced auxin signaling in root tips, LR primordia, and epidermal cells. Both lrlp1 and yuc8 mutants showed diminished IAA concentration responses compared to WT under both P conditions. Transcriptomic profiling demonstrated that both mutants exhibit blunted induction of P-starvation response (PSR) genes and auxin signaling genes, including phosphate transporters (OsPHT1s), phosphate signaling components (OsSPXs), and Auxin Response Factors (OsARFs), linking auxin biosynthesis and signaling to PSR regulation. Our work uncovers a conserved yet diversified auxin biosynthesis module that shapes RSA plasticity under P stress, with OsTAR2 adopting a broader regulatory role than its Arabidopsis homologs and downstream gene OsYUC8 in rice. These findings provide actionable targets for breeding P-efficient rice through precision engineering of auxin-mediated root adaptations.
- 相关文献
作者其他论文 更多>>
-
Inhibition of the invasive plant Ambrosia trifida by Sigesbeckia glabrescens extracts
作者：Yin, Lijuan;Zhang, Yuxin;Wangchen, Jiaozi;Wan, Fanghao;Liu, Bo;Qian, Wanqiang;Yin, Lijuan;Zhang, Guangzhong;Zhao, Haoyu
关键词：Ambrosia trifida; Sigesbeckia glabrescens extracts; Decreased carbon and nitrogen metabolism; Oxidative stress; Major allelopathic metabolites
-
Toxoplasma gondii Type I TR and ROP16 Synergistically Downregulate IL-12 to Inhibit Host Reactive Oxygen Species Production
作者：Geng, Xiaoling;Li, Ruifang;Du, Jingying;Zhang, Manyu;Jiang, Wei;Sun, Qing;Mi, Rongsheng;Qin, Shuang;Wang, Quan;Geng, Xiaoling
关键词：
Toxoplasma gondii ; CRISPR/Cas9; thioredoxin reductase; rhoptry protein 16; immune escape; oxidative stress -
Key events in the process of sex determination and differentiation in early chicken embryos
作者：Lv, Xiaoqian;Sun, Changhua;Liu, Xin;Liu, Guanzheng;Gong, Wei;Qian, Hongwu;Li, Zeyu;Wu, Jun;Zhu, Xilin;Niu, Yingjie;Sun, Hongyan;Chen, Guo hong;Jin, Kai;Li, Bichun;Zuo, Qisheng;Lv, Xiaoqian;Sun, Changhua;Liu, Xin;Liu, Guanzheng;Gong, Wei;Qian, Hongwu;Li, Zeyu;Wu, Jun;Zhu, Xilin;Niu, Yingjie;Sun, Hongyan;Chen, Guo hong;Jin, Kai;Li, Bichun;Zuo, Qisheng;Sun, Changhua;Chen, Guo hong;Li, Bichun;Song, Jiuzhou;Han, Wei;Li, Bichun
关键词：Chicken; DNA Methylation; Glycolysis; Histone Acetylation; Sex Determination
-
An Inducible CYP19A1 Excision Model for Sexual Differentiation in Chicken (Gallus gallus) via the CRISPR/Cas9 System
作者：Liu, Guangzheng;Peng, Yixiu;Li, Zeyu;Zhu, Xilin;Sun, Hongyan;Chen, Guohong;Zuo, Qisheng;Niu, Yingjie;Li, Bichun;Jin, Kai;Liu, Guangzheng;Peng, Yixiu;Li, Zeyu;Zhu, Xilin;Sun, Hongyan;Chen, Guohong;Zuo, Qisheng;Niu, Yingjie;Li, Bichun;Jin, Kai;Sun, Hongyan;Chen, Guohong;Zuo, Qisheng;Niu, Yingjie;Jin, Kai;Song, Jiuzhou;Han, Wei;Jin, Kai;Wei, Wanhong;Li, Bichun
关键词：chickens; gene knockout; gender control; CRISPR/Cas9; Tet-on
-
Protein Prenylation in Plants: Mechanisms and Functional Implications
作者：Tian, Chang;Wang, Quan
关键词：protein prenylation; farnesyltransferase; geranylgeranyltransferase; modification mechanism; functional implications
-
CRISPR-Cas9-mediated knockout of OsKCS11 in rice reveals potential crosstalk between very-long-chain fatty acids and cytokinin
作者：Zhou, Jianping;Guo, Yachong;Wang, Yibo;Yang, Hongjun;Wang, Wei;Fu, Xinxuan;Zhang, Rui;Zhang, Yong;Pang, Rui;Wang, Quan;Han, Yangshuo;Zheng, Xuelian;Zhang, Yong;Han, Yangshuo;Zhang, Tao
关键词：very-long-chain fatty acid; beta-ketoacyl-CoA synthase; CRISPR-Cas9; acetyl-CoA; cytokinin; rice
-
Generation of chicken induced pluripotent stem cells (iPSCs) from somatic cells using multi-lentiviral transduction and OAC2
作者：Liu, Guangzheng;Zhu, Xilin;Li, Zeyu;Zhu, Liqian;Chen, Guohong;Niu, Yingjie;Sun, Hongyan;Zuo, Qisheng;Li, Bichun;Jin, Kai;Liu, Guangzheng;Zhu, Xilin;Li, Zeyu;Zhu, Liqian;Chen, Guohong;Niu, Yingjie;Sun, Hongyan;Zuo, Qisheng;Li, Bichun;Jin, Kai;Chen, Guohong;Niu, Yingjie;Sun, Hongyan;Zuo, Qisheng;Jin, Kai;Song, Jiuzhou;Han, Wei;Jin, Kai;Wei, Wanhong;Li, Bichun
关键词：Chicken; iPSCs; Somatic cell reprogramming; Multi-lentiviral; OAC2
