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Characterization of SHATTERPRROOF Homoeologs and CRISPR-Cas9-Mediated Genome Editing Enhances Pod-Shattering Resistance in Brassica napus L.

文献类型： 外文期刊

作者： Zaman, Qamar U. 1 ; Chu Wen 1 ; Shi Yuqin 1 ; Hao Mengyu 1 ; Mei Desheng 1 ; Jacqueline, Batley 3 ; Zhang Baohong 4 ; Li 1 ;

作者机构： 1.Chinese Acad Agr Sci, Oil Crops Res Inst, Key Lab Biol & Genet Improvement Oil Crops, Minist Agr & Rural Affairs, 2 Xudong 2nd Rd, Wuhan 430062, Peoples R China

2.Chinese Acad Agr Sci, Grad Sch, Beijing, Peoples R China

3.Univ Western Australia, Sch Biol Sci, Perth, WA, Australia

4.East Carolina Univ, Dept Biol, Greenville, NC 27858 USA

期刊名称：CRISPR JOURNAL （ 影响因子：6.071； 五年影响因子：6.071 ）

ISSN： 2573-1599

年卷期： 2021 年 4 卷 3 期

页码：

收录情况： SCI

摘要： Brassica napus is the most important oil crop plant for edible oil and renewable energy source worldwide. Yield loss caused by pod shattering is a main problem during B. napus harvest. In this study, six BnSHP1 and two BnSHP2 homoeologs were targeted by the CRISPR (clustered regularly interspaced short palindromic repeats)-Cas9 (CRISPR-associated protein 9) genome editing system and multiple SHP1 and SHP2 mutated lines were identified for evaluating the contribution for pod-shattering resistance. Our data suggest that BnSHP1A09 is probably a promising homoeolog for controlling lignin contents at dehiscence zone. Simultaneous mutation of BnSHP1A09/C04-B/A04 and BnSHP2A05/C04-A exhibited reduced lignified layer and separation layer adjacent to valves and replum. The pod-shattering resistance index (SRI) subsequently increased to 0.31 in five homoeolog mutation lines compared with the wild type (SRI= 0.036), which provide the theoretical basis for breeding of commercial pod-shattering resistance variety.

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