Ribosome Pausing Negatively Regulates Protein Translation in Maize Seedlings during Dark-to-Light Transitions
文献类型： 外文期刊
作者： Hou, Mingming 1 ; Fan, Wei 1 ; Zhong, Deyi 2 ; Dai, Xing 3 ; Wang, Quan 1 ; Liu, Wanfei 1 ; Li, Shengben 1 ;
作者机构： 1.Chinese Acad Agr Sci, Agr Genom Inst Shenzhen, Shenzhen Branch, Genome Anal Lab,Minis Agr & Rural Affairs,Guangdon, Shenzhen 518120, Peoples R China
2.Nanjing Agr Univ, Acad Adv Interdisciplinary Studies, Nanjing 210095, Peoples R China
3.Shenzhen Univ, Longhua Bioind & Innovat Res Inst, Coll Life Sci & Oceanog, Guangdong Prov Key Lab Plant Epigenet, Shenzhen 518060, Peoples R China
关键词： translational regulation; Ribo-seq; ribosome pausing; maize; translatomics
期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：4.9； 五年影响因子：5.6 ）
ISSN： 1661-6596
年卷期： 2024 年 25 卷 14 期
页码：
收录情况： SCI
摘要： Regulation of translation is a crucial step in gene expression. Developmental signals and environmental stimuli dynamically regulate translation via upstream small open reading frames (uORFs) and ribosome pausing. Recent studies have revealed many plant genes that are specifically regulated by uORF translation following changes in growth conditions, but ribosome-pausing events are less well understood. In this study, we performed ribosome profiling (Ribo-seq) of etiolated maize (Zea mays) seedlings exposed to light for different durations, revealing hundreds of genes specifically regulated at the translation level during the early period of light exposure. We identified over 400 ribosome-pausing events in the dark that were rapidly released after illumination. These results suggested that ribosome pausing negatively regulates translation from specific genes, a conclusion that was supported by a non-targeted proteomics analysis. Importantly, we identified a conserved nucleotide motif downstream of the pausing sites. Our results elucidate the role of ribosome pausing in the control of gene expression in plants; the identification of the cis-element at the pausing sites provides insight into the mechanisms behind translation regulation and potential targets for artificial control of plant translation.
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