HS-GC-IMS couples with convolutional neural network for Burkholderia gladioli pv. Cocovenenans detection in Auricularia Auricula
文献类型: 外文期刊
作者: Niu, Chen 1 ; Zhao, Mincheng 2 ; Sheng, Qinglin 1 ; Wu, Yuanchun 1 ; Song, Zihan 3 ; Wei, Jianping 1 ; Yuan, Yahong 1 ; Yue, Tianli 1 ;
作者机构: 1.Northwest Univ, Coll Food Sci & Technol, Xian 710069, Peoples R China
2.China Elect Technol Grp Corp 20th Res Inst, Beijing 710068, Peoples R China
3.Chinese Acad Agr Sci, Inst Vegetables & Flowers, Beijing 100081, Peoples R China
关键词: Bongkrekic acid; HS-GC-IMS; Deep learning; Detection; Burkholderia gladioli pv. Cocovenenans
期刊名称:FOOD CHEMISTRY ( 影响因子:9.8; 五年影响因子:9.7 )
ISSN: 0308-8146
年卷期: 2025 年 486 卷
页码:
收录情况: SCI
摘要: The shortage in early detection methods for the pathogen Burkholderia gladioli pv. cocovenenans (BGC) and its toxin bongkrekic acid rises the risk for food poisoning. Combining Headspace-Gas Chromatography-Ion Mobility Spectrometry (HS-GC-IMS) with convolutional neural network, we established a HS-GC-IMS-VGGNet architecture for the detection of BGC in edible fungus Auricularia auricula (AA) contaminated by six microorganisms species and the overall accuracy was 93.8 %. Meanwhile, the network achieved a limit of detection (LOD) of 80 CFU/mL and limit of quantification (LOQ) of 241 CFU/mL for BGC biomass. An LOD of 0.25 mg/L for bongkrekic acid detection covering the range of 0-1.54 mg/kg was achieved directly in the AA matrix. Furthermore, 28 microbial organic volatiles were extracted by Gradient-weighted Class Activation Mapping (Grad-CAM) and identified as conducive to the BKA detection. In all, the detection system established for BGC and its bongkrekic acid toxin is of good accuracy, precision and possesses greener mode.
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