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Structural analysis and immunological activity of a novel low molecular weight neutral polysaccharide isolated from Hemerocallis citrina Borani

文献类型: 外文期刊

作者: Ti, Yongrui 1 ; Zhang, Yanli 1 ; Hou, Yuqing 1 ; Ban, Yuqian 1 ; Wang, Xiaoxiao 1 ; Li, Guoliang 1 ; Song, Zihan 1 ;

作者机构: 1.Chinese Acad Agr Sci, Inst Vegetables & Flowers, State Key Lab Vegetable Biobreeding, Beijing 100081, Peoples R China

关键词: Hemerocallis citrina Borani; Purification; Low molecular weight neutral polysaccharide; Structural analysis; Immunological effect

期刊名称:FOOD CHEMISTRY ( 影响因子:9.8; 五年影响因子:9.7 )

ISSN: 0308-8146

年卷期: 2025 年 469 卷

页码:

收录情况: SCI

摘要: A novel neutral water-soluble polysaccharide (HCBP1-1) was isolated and purified from Hemerocallis citrina Borani by column chromatography. The fine structure of HCBP1-1 was determined by a series of physical and chemical means. HCBP1-1 was a homogeneous low molecular weight polysaccharide of 7.25 kDa. Methylation and NMR analysis revealed that HCBP1-1 consisted of alpha-D-Glcp-(1-*,-*4)-alpha-D-Glcp-(1-*,-*4)-beta-D-Galp-(1-*,-*4,6)-alpha-D-Glcp-(1-*,-*3,4)-alpha-D-Glcp-(1-*. In particular, the main backbone of HCBP1-1 consisted of unsubstituted (4-alpha-D-Glcp and 4-beta-D-Galp) and monosubstituted (4,6-alpha-D-Glcp and 3,4-alpha-D-Glcp) glucose and galactose units linked by alpha-(1-* 4) and beta-(1-* 4) glycosidic linkages. The side chain consisted of alpha-D-Glcp-(1-* and-*4)alpha-D-Glcp-(1-*. Furthermore, bioactivity assays show that HCBP1-1 significantly promoted the secretion of NO, TNF-alpha, IL-1 beta, and IL-6 and the expression of NF-kappa B p65 in RAW264.7 cells, which had good immune-enhancing activity. These results suggest that HCBP1-1 had beneficial immunomodulatory effects and could be developed into functional foods or nutraceuticals with immune-enhancing properties.

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