β-Lactoglobulin separation from whey protein: A comprehensive review of isolation and purification techniques and future perspectives
文献类型： 外文期刊
作者： Wang, Z. L. 1 ; Tang, X. 1 ; Wang, M. 2 ; She, Y. X. 2 ; Yang, B. R. 3 ; Sheng, Q. H. 1 ; Abd El-Aty, A. M. 4 ;
作者机构： 1.Hebei Agr Univ, Sch Food Sci & Technol, Baoding 07100, Peoples R China
2.Chinese Acad Agr Sci, Inst Qual Standardizat & Testing Technol Agroprod, Beijing 100081, Peoples R China
3.Univ Turku, Fac Technol, Dept Life Technol, Food Sci, FI-20014 Turku, Finland
4.Cairo Univ, Fac Vet Med, Dept Pharmacol, Giza 12211, Egypt
5.Ataturk Univ, Med Fac, Dept Med Pharmacol, TR-25240 Erzurum, Turkiye
关键词： milk allergy; allergy mechanism; (3-lactoglobulin; separation and purification techniques; hypoallergenic dairy
期刊名称：JOURNAL OF DAIRY SCIENCE （ 影响因子：4.4； 五年影响因子：4.4 ）
ISSN： 0022-0302
年卷期： 2024 年 107 卷 12 期
页码：
收录情况： SCI
摘要： Cow milk, although rich in essential nutrients, is a wellknown food allergen that can cause allergic reactions in infants and young children. (3-Lactoglobulin accounts for 10% of the total protein in milk and 50% of the whey protein, which has high nutritional value and excellent functional properties but is also the main allergen leading to milk protein allergy. Exploring the mechanism of milk allergy and selecting suitable separation and purification methods to obtain high-purity (3-LG is the premise of research on reducing allergenicity. In this review, the research progress in membrane technology, gel filtration chromatography, ion exchange chromatography, affinity chromatography, precipitation, and aqueous 2-phase system separation for the separation and purification of milk (3-LG is reviewed in detail to promote the further development of milk (3-LG separation and purification methods and provide a new method for the development of hypoallergenic dairy products in the future. Among these methods, ion exchange chromatography and gel chromatography are widely used, precipitation is generally used as a crude purification step, and HPLC and membrane technology are used for further purification to improve the purity of allergens.
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