文献类型： 外文期刊

作者： Yin, Cheng ¹ ; OReilly, Andrias O. ³ ; Liu, Shao-Nan ¹ ; Du, Tian-Hua ¹ ; Gong, Pei-Pan ¹ ; Zhang, Cheng-Jia ⁴ ; Wei, Xue-Gao ¹ ; Yang, Jing ¹ ; Huang, Ming-Jiao ¹ ; Fu, Bu-Li ¹ ; Liang, Jin-Jin ¹ ; Xue, Hu ¹ ; Hu, Jin-Yu ¹ ; Ji, Yao ¹ ; He, Chao ¹ ; Du, He ¹ ; Wang, Chao ¹ ; Zhang, Rong ¹ ; Tan, Qi-Mei ⁴ ; Lu, Han-Tang ¹ ; Xie, Wen ¹ ; Chu, Dong ⁵ ; Zhou, Xu-Guo ⁶ ; Nauen, Ralf ⁷ ; Gui, Lian-You ² ; Bass, Chris ⁸ ; Yang, Xin ¹ ; Zhang, You-Jun ¹ ;

作者机构： 1.Inst Vegetables & Flowers, Chinese Acad Agr Sci, State Key Lab Vegetable Biobreeding, Beijing, Peoples R China

2.Yangtze Univ, Coll Agr, Hubei Engn Technol Ctr Pest Forewarning & Manageme, Jingzhou, Hubei, Peoples R China

3.Liverpool John Moores Univ, Sch Biol & Environm Sci, Liverpool, England

4.Hunan Agr Biotechnol Res Inst, Hunan Prov Key Lab Pesticide Biol & Precise Use Te, Changsha, Peoples R China

5.Qingdao Agr Univ, Sch Agr & Plant Protect, Key Lab Integrated Crop Pest Management Shandong P, Qingdao, Shandong, Peoples R China

6.Univ Kentucky, Dept Entomol, Lexington, KY USA

7.Crop Sci Div, Bayer AG, R&D, Monheim, Germany

8.Univ Exeter, Ctr Ecol & Conservat, Penryn Campus, Penryn, Cornwall, England

期刊名称：PLOS GENETICS （ 影响因子：4.5； 五年影响因子：5.5 ）

ISSN： 1553-7404

年卷期： 2024 年 20 卷 2 期

页码：

收录情况： SCI

摘要： Neonicotinoid insecticides, which target insect nicotinic acetylcholine receptors (nAChRs), have been widely and intensively used to control the whitefly, Bemisia tabaci, a highly damaging, globally distributed, crop pest. This has inevitably led to the emergence of populations with resistance to neonicotinoids. However, to date, there have been no reports of target-site resistance involving mutation of B. tabaci nAChR genes. Here we characterize the nAChR subunit gene family of B. tabaci and identify dual mutations (A58T&R79E) in one of these genes (BT beta 1) that confer resistance to multiple neonicotinoids. Transgenic D. melanogaster, where the native nAChR D beta 1 was replaced with BT beta 1(A58T&R79E), were significantly more resistant to neonicotinoids than flies where D beta 1 were replaced with the wildtype BT beta 1 sequence, demonstrating the causal role of the mutations in resistance. The two mutations identified in this study replace two amino acids that are highly conserved in >200 insect species. Three-dimensional modelling suggests a molecular mechanism for this resistance, whereby A58T forms a hydrogen bond with the R79E side chain, which positions its negatively-charged carboxylate group to electrostatically repulse a neonicotinoid at the orthosteric site. Together these findings describe the first case of target-site resistance to neonicotinoids in B. tabaci and provide insight into the molecular determinants of neonicotinoid binding and selectivity.