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Co-overexpression of ZlRc and ZlRd increases flavonoid content, antioxidant activity, and inhibitory effects on against α-glucosidase, α-amylase, pancreatic lipase, and tyrosinase without affecting rice agronomic traits or yield

文献类型： 外文期刊

作者： Ma, Qing 1 ; Li, Wanhong 1 ; Wang, Lixia 1 ; Zhang, Hongbo 1 ; Zhang, Zhongfeng 1 ; Yan, Ning 1 ;

作者机构： 1.Chinese Acad Agr Sci, Tobacco Res Inst, Key Lab Synthet Biol, Minist Agr & Rural Affairs, Qingdao 266101, Peoples R China

2.Chinese Acad Agr Sci ECS CAAS, East China Agritech Ctr, Suzhou 215331, Peoples R China

3.Chinese Acad Agr Sci, Grad Sch, Beijing 100081, Peoples R China

关键词： Co-overexpression; Agronomic traits; Antioxidant activity; Flavonoid; Transcriptome

期刊名称：PLANT PHYSIOLOGY AND BIOCHEMISTRY （ 影响因子：5.7； 五年影响因子：6.4 ）

ISSN： 0981-9428

年卷期： 2025 年 227 卷

页码：

收录情况： SCI

摘要： Pigmented rice (e.g., black or purple rice), rich in flavonoids and other active compounds, has lower yields than non-pigmented rice. The study aimed to breed pigmented rice with stable yields. To this end, we created transgenic rice by inserting the ZlRc and ZlRd genes from Chinese wild rice (Zizania latifolia) into rice (Oryza sativa) and investigated the resulting yield and flavonoid content. Rc encodes a transcription factor that regulates flavonoid biosynthesis, while dihydroflavonol 4-reductase (DFR/Rd) is an important flavonoid biosynthetic enzyme. The dark brown colour of the pericarp rice was attributed to the co-overexpression of ZlRc and ZlRd (ZlRcRd), which did not influence the agronomic traits or yield. No significant difference was observed in the chlorophyll content or photosynthetic gas exchange parameters between the ZlRcRd and wild-type (WT) rice. The flavonoid content in the ZlRcRd rice seeds was significantly enhanced, and so were antioxidant activity and inhibitory effects on alpha-glucosidase, alpha-amylase, pancreatic lipase, and tyrosinase. Compared with WT rice, 1080 genes and 82 flavonoids were upregulated in the ZlRcRd rice. The expression of key genes involved in flavonoid biosynthesis (CHS, F3 ' H, and F3 ' 5 ' H) and the activities of related key enzymes were significantly higher in ZlRcRd than WT rice. This study proposes new germplasm resources and technical approaches for breeding pigmented rice with stable yields and high flavonoid content, which will address the challenge of low yields of pigmented rice and increase farmers' enthusiasm for planting pigmented rice.

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