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The Metacaspase Gene PoMCA1 Enhances the Mycelial Heat Stress Tolerance and Regulates the Fruiting Body Development of Pleurotus ostreatus

文献类型： 外文期刊

作者： Pei, Jingqi 1 ; Zhao, Mengran 2 ; Zhang, Lijiao 2 ; Wu, Xiangli 2 ;

作者机构： 1.Shanxi Agr Univ, Coll Food Sci & Engn, Jinzhong 030801, Peoples R China

2.Chinese Acad Agr Sci, Inst Agr Resources & Reg Planning, State Key Lab Efficient Utilizat Arid & Semiarid A, Beijing 100081, Peoples R China

3.Minist Agr & Rural Affairs, Key Lab Microbial Resources, Beijing 100081, Peoples R China

关键词： fruiting body development; genetic transformation; heat stress; metacaspase; Pleurotus ostreatus; qRT-PCR

期刊名称：HORTICULTURAE （ 影响因子：3.1； 五年影响因子：3.4 ）

ISSN：

年卷期： 2024 年 10 卷 2 期

页码：

收录情况： SCI

摘要： Pleurotus ostreatus is one of the most cultivated edible mushrooms worldwide, of which the fruiting body development is a highly complex process involving the precise genetic regulatory network and suitable environmental factors. Metacaspases play important roles in developmental processes and programmed cell death (PCD) induced by some environmental stress in many organisms. In this study, a type I metacaspase, PoMCA1, was identified via the analysis of the enzyme domain and alignment with homologous metacaspases. PoMCA1 overexpression and RNAi mutants were generated via Agrobacterium tumefaciens-mediated transformation (ATMT) into the P. ostreatus mycelium. The roles of the PoMCA1 gene in heat stress and fruiting body development were examined. The results show that both of the overexpression transformants were more tolerant to heat stress than the wild-type strain, while the opposite phenomenons were found for the two RNAi strains. Compared with the wild-type strain, the overexpression strain OE-7 had faster formation of the fruiting body, while the two RNAi strains produced significantly more primordia and young fruiting bodies, and presented morphological deformities and slower fruiting body development. All of the results suggest that the PoMCA1 gene is involved in the positive regulation of heat stress tolerance and fruiting body development in P. ostreatus.

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