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Effect of combined cold pretreatment and heat shock on microspore cultures in broccoli

文献类型： 外文期刊

作者： Yuan, S. X. 1 ; Liu, Y. M. 1 ; Fang, Z. Y. 1 ; Yang, L. M. 1 ; Zhuang, M. 1 ; Zhang, Y. Y. 1 ; Sun, P. T. 1 ;

作者机构： 1.Chinese Acad Agr Sci, Inst Vegetables & Flowers, Beijing 100081, Peoples R China

关键词： genotype;embryogenesis;cold pretreatment;diploid frequency

期刊名称：PLANT BREEDING （ 影响因子：1.832； 五年影响因子：1.956 ）

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收录情况： SCI

摘要： P>Applied stress is often used to induce embryogenesis in microspore culture. Cold pretreatment has been used in cereal crops, Brassica napus and Brassica rapa microspore cultures, but seldom attempted in Brassica oleracea microspore culture. In this study, the effect of cold pretreatment (4 degrees C) on embryogenesis in microspore culture and ploidy of regenerants resulting from those cultures was investigated in three broccoli cultivars 'Green Elite', 'Green Star' and 'TI-111'. Cold pretreatment for 1, 2 or 3 days was effective when applied alone using these three cultivars, but the frequency of embryos derived from microspores was low. The combination of cold pretreatment (4 degrees C) for 1 or 2 days and heat shock (32.5 degrees C) for 1 day significantly enhanced microspore embryogenesis efficiency, especially with 'TI-111' for which it was increased by 63-72-fold. In addition, the combined treatment resulted in higher diploid frequency of the regenerated population compared to traditional microspore culture protocol which typical only uses heat shock at 32.5 degrees C for 1 day in broccoli.

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