Application of high-throughput sequencing for microbial diversity detection in feces of specific-pathogen-free ducks
文献类型： 外文期刊
作者： Zhao, L. L. 1 ; Yin, H. C. 1 ; Lu, T. F. 1 ; Niu, Y. J. 1 ; Zhang, Y. Y. 1 ; Li, S. Q. 2 ; Wang, Y. P.; Chen, H. Y.;
作者机构： 1.Chinese Acad Agr Sci, Harbin Vet Res Inst, Heilongjiang Prov Key Lab Lab Anim & Comparat Med, State Key Lab Vet Biotechnol, 678 Haping Rd, Harbin 150069, Heilongjiang, Peoples R China
2.Chinese Acad Agr Sci, Harbin Vet Res Inst, Heilongjiang Prov Key Lab Lab Anim & Comparat Med, State
关键词： feces; high-throughput sequencing; microbial diversity; SPF ducks
期刊名称：POULTRY SCIENCE （ 影响因子：3.352； 五年影响因子：3.679 ）
ISSN： 0032-5791
年卷期： 2018 年 97 卷 7 期
页码：
收录情况： SCI
摘要： High-throughput sequencing technologies play important roles in the study of animal enteric microorganisms. Fecal samples collected from 3 strains (9 samples) of specific-pathogen-free (SPF) ducks raised in an isolator. Duck intestinal bacterial flora were analyzed by sequencing distinct regions of the 16S rRNA genes using an Illumina HiSeq2500 platform. The result showed the most abundant primary enteric microbial phyla of 3 different strains of 70-week-old SPF ducks were Firmicutes, Proteobacteria, and Bacteroidetes, the most abundant primary enteric microbial order were Clostridiales, Lactobacillales, and Aeromonadales, and the most abundant primary enteric microbial genera were Bacteroides, Comamonas, and Enterococcus. In addition, the 3 duck strains harbored different compositions of the microorganisms, but these differences were not significant. Duck intestinal bacterial flora were analyzed using a high-throughput sequencing approach to further understand the distribution of intestinal flora and the biology of SPF ducks to ultimately benefit the purification of SPF ducks.
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