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Transcriptional and functional analysis of epilactose transport and catabolism in the probiotic Ligilactobacillus salivarius ZL6

文献类型： 外文期刊

作者： Zhang, Yuwei 1 ; Xu, Xiaoqing 1 ; Peng, Qing 1 ; Tian, Yanting 2 ; Chen, Xiaoming 2 ; Tian, Dandan 1 ; Ma, Lan 1 ; Gong, Yuxin 1 ; Qiao, Yu 1 ; Shi, Bo 1 ;

作者机构： 1.Chinese Acad Agr Sci, Feed Res Inst, 12 Zhongguancun South St, Beijing 100081, Peoples R China

2.Tianjin Acad Agr Sci, Inst Agroprod Preservat & Proc Technol, Tianjin 300380, Peoples R China

关键词： Ligilactobacillus salivarius ZL6; Epilactose; LacS; LacZ; Prebiotic metabolism

期刊名称：FOOD BIOSCIENCE （ 影响因子：5.9； 五年影响因子：6.1 ）

ISSN： 2212-4292

年卷期： 2025 年 63 卷

页码：

收录情况： SCI

摘要： The ability of probiotics to metabolize host-derived compounds is critical for their survival in the human and animal gut. Prebiotics can enhance probiotic growth or persistence, both of them forming synergistic synbiotics. Epilactose (4-O-(3-D-galactopyranosyl-D-mannose) is a promising prebiotic that can promote the growth and activity of beneficial Lactobacilli and Bifidobacteria in the intestine. This study aimed to identify the genes involved in epilactose transport and catabolism in Ligilactobacillus salivarius ZL6. The RNA sequencing (RNA-seq) analysis revealed that epilactose increase in the expression of lacS, encoding a galactoside-pentose-hexuronide (GPH) permease, within the gal-lac gene cluster. Additionally, lacZ, encoding (3-galactosidase, and other genes in the Leloir pathway were up-regulated in response to epilactose. Functional analysis of lacS and lacZ mutants confirmed that epilactose transport depended on the permease encoded by lacS, and its degradation into galactose and mannose was mediated by (3-galactosidase encoded by lacZ. A second, unidentified (3-galactosidase may also play a role in epilactose metabolism. The results in this research can provide some valuable insights into understanding epilactose's transport and catabolism pathways in Ligilactobacillus salivarius ZL6 and better support for demonstrating the prebiotic activity of epilactose.

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