Heterologous Expression of Platycodon grandiflorus PgF3′5′H Modifies Flower Color Pigmentation in Tobacco
文献类型： 外文期刊
作者： Ma, Lulin 1 ; Jia, Wenjie 1 ; Duan, Qing 1 ; Du, Wenwen 1 ; Li, Xiang 1 ; Cui, Guangfen 1 ; Wang, Xiangning 1 ; Wang, Jihua 1 ;
作者机构： 1.Yunnan Acad Agr Sci, Natl Engn Res Ctr Ornamental Hort, Key Lab Yunnan Flower Breeding, Flower Res Inst, Kunming 650205, Peoples R China
关键词： PgF3'5'H; transgenic tobacco; over-expression; delphinidin-based anthocyanin; blue flower color
期刊名称：GENES （ 影响因子：3.5； 五年影响因子：3.9 ）
ISSN：
年卷期： 2023 年 14 卷 10 期
页码：
收录情况： SCI
摘要： Flavonoid-3 ',5 '-hydroxylase (F3 ' 5 ' H) is the key enzyme for the biosynthesis of delphinidin-based anthocyanins, which are generally required for purple or blue flowers. Previously, we isolated a full-length cDNA of PgF3 ' 5 ' H from Platycodon grandiflorus, which shared the highest homology with Campanula medium F3 ' 5 ' H. In this study, PgF3 ' 5 ' H was subcloned into a plant over-expression vector and transformed into tobacco via Agrobacterium tumefaciens to investigate its catalytic function. Positive transgenic tobacco T-0 plants were obtained by hygromycin resistance screening and PCR detection. PgF3 ' 5 ' H showed a higher expression level in all PgF3 ' 5 ' H transgenic tobacco plants than in control plants. Under the drive of the cauliflower mosaic virus (CaMV) 35S promoter, the over-expressed PgF3 ' 5 ' H produced dihydromyricetin (DHM) and some new anthocyanin pigments (including delphinidin, petunidin, peonidin, and malvidin derivatives), and increased dihydrokaempferol (DHK), taxifolin, tridactyl, cyanidin derivatives, and pelargonidin derivatives in PgF3 ' 5 ' H transgenic tobacco plants by ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) analysis, resulting in a dramatic color alteration from light pink to magenta. These results indicate that PgF3 ' 5 ' H products have F3 ' 5 ' H enzyme activity. In addition, PgF3 ' 5 ' H transfer alters flavonoid pigment synthesis and accumulation in tobacco. Thus, PgF3 ' 5 ' H may be considered a candidate gene for gene engineering to enhance anthocyanin accumulation and the molecular breeding project for blue flowers.
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