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HvStaufenC contributes to the high RNAi efficiency in the 28-spotted ladybeetle, Henosepilachna vigintioctopunctata

文献类型： 外文期刊

作者： Li, Zhaoyang 1 ; Yoon, June-Sun 2 ; Zhong, Zexin 1 ; Ruan, Yalin 1 ; Yang, Chunxiao 1 ; Zhou, Xuguo 3 ; Zhang, Youjun 4 ; Pan, Huipeng 1 ;

作者机构： 1.South China Agr Univ, Minist Educ, Engn Res Ctr Biocontrol, State Key Lab Green Pesticide, Guangzhou 510642, Peoples R China

2.Jeonbuk Natl Univ, Dept Agr Convergence Technol, Jeonbuk 54896, South Korea

3.Univ Illinois, Dept Entomol, Urbana, IL 61801 USA

4.Chinese Acad Agr Sci, Inst Vegetables & Flowers, State Key Lab Vegetable Biobreeding, Beijing 100081, Peoples R China

关键词： RNAi efficiency; CRISPR/Cas9; StaufenC; Henosepilachna vigintioctopunctata

期刊名称：INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY （ 影响因子：3.7； 五年影响因子：4.1 ）

ISSN： 0965-1748

年卷期： 2025 年 180 卷

页码：

收录情况： SCI

摘要： RNA interference (RNAi) has been shown to be relatively effective in coleopteran insects, with limited exploration into the molecular mechanisms that underlie this effectiveness. This study specifically examines the 28-spotted ladybeetle, Henosepilachna vigintioctopunctata (Hvig), known for its high RNAi efficiency. Here, we utilized RNAi and CRISPR/Cas9 techniques to identify and validate the genes involved in the RNAi pathway that enhance RNAi efficacy in Hvig. We identified a total of 15 potential genes within the RNAi pathway that may impact RNAi efficiency. The bioassay results showed that only knockdown of HvStaufenC in the 3rd instar larvae could block the abnormal body color phenotype and lethality induced by the subsequent silencing of the two marker genes, HvTH (tyrosine hydroxylase) and HvABCH1 (ATP-binding cassette H transporter gene), respectively. Additionally, successful CRISPR/Cas9-mediated knockout of HvStaufenC led to the generation of stable, heritable mutants that exhibited insensitivity to RNAi, displaying no response to RNAi targeting HvTH and HvABCH1. Compared to the wild-type strain, the HvStaufenC knockout (HvStaufenCKO) mutant females demonstrated a 42 % decrease in oviposition rate and a 41.3 % reduction in egg hatchability. This study demonstrates that HvStaufenC gene is crucial for the RNAi efficiency of Hvig and offers new evidence into the RNAi mechanisms in coleopteran species.

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