Structure-function analysis of MhieJHAMT: a key enzyme in Monolepta hieroglyphica juvenile hormone biosynthesis
文献类型： 外文期刊
作者： Song, Xuan 1 ; Zhang, Da-wei 4 ; Yang, Yong 3 ; Kou, Jun-feng 6 ; Liu, Chang 7 ; Yi, Chao-qun 2 ; Tang, Zi-yi 2 ; Liu, Wen-tao 2 ; Chen, Run 2 ; Guo, Wei-hao 2 ; Zhang, Tian-tao 2 ; Zhang, Yong-jun 1 ;
作者机构： 1.Chinese Acad Agr Sci, Zhongyuan Res Ctr, Xinxiang 453500, Henan, Peoples R China
2.Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China
3.Zhangye Acad Agr Sci, Inst Plant Protect, Zhangye 734000, Gansu, Peoples R China
4.Gansu Acad Agr Sci, Inst Plant Protect, Lanzhou 730070, Gansu, Peoples R China
5.Chinese Acad Agr Sci, Inst Plant Protect, Natl Key Lab Integrated Management Crop Dis & Pest, Beijing 100193, Peoples R China
6.CangZhou Acad Agr & Forestry Sci, Cangzhou 061000, Heibei, Peoples R China
7.Ningxia Acad Agr & Forestry Sci, Inst Plant Protect, Yinchuan 750002, Ningxia, Peoples R China
8.Huazhong Agr Univ, Coll Plant Sci & Technol, Wuhan 430070, Hubei, Peoples R China
9.Agr Univ Hebei, Coll Plant Protect, Baoding 071000, Hebei, Peoples R China
关键词： Monolepta hieroglyphica; Coleoptera pest; Juvenile hormone acid O-methyltransferase; Molecular dynamics; RNAi
期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：8.5； 五年影响因子：8.7 ）
ISSN： 0141-8130
年卷期： 2025 年 318 卷
页码：
收录情况： SCI
摘要： Monolepta hieroglyphica, a major pest of Chinese crops, demands effective control. Targeting juvenile hormone acid O-methyltransferase, a key enzyme in JH production, presents a promising strategy for developing novel, specific insecticides. This study investigated the structure-function of M. hieroglyphica juvenile hormone acid Omethyltransferase (MhieJHAMT), a key enzyme in the JH pathway. Phylogenetic analysis showed MhieJHAMT's conservation within Coleoptera, suggesting a conserved catalytic mechanism. In vitro enzymatic activity assays confirmed MhieJHAMT's catalytic function. Bioinformatics (molecular dynamics and docking) revealed 19 amino acid residues forming the active site, binding S-adenosylmethionine (SAM) and farnesoic acid (FA). In vivo RNA interference (RNAi) coupled with ELISA demonstrated MhieJHAMT's crucial role in JH biosynthesis; its knockdown significantly reduced JH titers. Quantitative real-time PCR (RT-qPCR) revealed higher MhieJHAMT expression in female intestines and gonads, hinting at additional functions beyond JH biosynthesis. Interestingly, our findings reveal an intriguing correlation between host plant-mediated alterations in farnesyl pyrophosphate synthase 1 (MhieFPPS1) expression and that of MhieJHAMT, with MhieFPPS1 appearing to function upstream of MhieJHAMT. The observation suggests a potential positive feedback loop, hinting at a complex regulatory mechanism. This comprehensive study elucidates MhieJHAMT's function, its regulatory mechanisms, and its potential as a novel pest control target.
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