A new chromosome-scale genome of wild Brassica oleracea provides insights into the domestication of Brassica crops
文献类型: 外文期刊
作者: Ji, Gaoxiang 1 ; Long, Ying 1 ; Cai, Guangqin 1 ; Wang, Aihua 4 ; Yan, Guixin 1 ; Li, Hao 1 ; Gao, Guizhen 1 ; Xu, Kun 1 ; Huang, Qian 1 ; Chen, Biyun 1 ; Li, Lixia 1 ; Li, Feng 1 ; Nishio, Takeshi 3 ; Shen, Jinxiong 2 ; Wu, Xiaoming 1 ;
作者机构: 1.Chinese Acad Agr Sci, Key Lab Biol & Genet Improvement Oil Crops, Minist Agr & Rural Affairs, Oil Crops Res Inst, Wuhan, Peoples R China
2.Huazhong Agr Univ, Natl Key Lab Crop Genet Improvement, Wuhan, Peoples R China
3.Tohoku Univ, Grad Sch Agr Sci, 468-1 Aza Aoba,Aramaki,Aoba Ku, Sendai 9800845, Japan
4.Wuhan Acad Agr Sci, Wuhan Vegetable Res Inst, Wuhan, Peoples R China
关键词: Broccoli; cauliflower; crop domestication; pangenome; wild Brassica oleracea; WUSCHEL
期刊名称:JOURNAL OF EXPERIMENTAL BOTANY ( 影响因子:6.9; 五年影响因子:8.0 )
ISSN: 0022-0957
年卷期: 2024 年 75 卷 10 期
页码:
收录情况: SCI
摘要: The cultivated diploid Brassica oleracea is an important vegetable crop, but the genetic basis of its domestication remains largely unclear in the absence of high-quality reference genomes of wild B. oleracea. Here, we report the first chromosome-level assembly of the wild Brassica oleracea L. W03 genome (total genome size, 630.7 Mb; scaffold N50, 64.6 Mb). Using the newly assembled W03 genome, we constructed a gene-based B. oleracea pangenome and identified 29 744 core genes, 23 306 dispensable genes, and 1896 private genes. We re-sequenced 53 accessions, representing six potential wild B. oleracea progenitor species. The results of the population genomic analysis showed that the wild B. oleracea populations had the highest level of diversity and represents the most closely related population to modern-day horticultural B. oleracea. In addition, the WUSCHEL gene was found to play a decisive role in domestication and to be involved in cauliflower and broccoli curd formation. We also illustrate the loss of disease-resistance genes during selection for domestication. Our results provide new insights into the domestication of B. oleracea and will facilitate the future genetic improvement of Brassica crops. A new chromosome-level assembly of the wild Brassica oleracea W03 genome provides insights into the speciation of Brassica crops and identifies WUSCHEL as a key gene in domestication.
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