文献类型： 外文期刊

作者： Sun, Huarun ¹ ; Zhang, Jingao ¹ ; Li, Xiaolu ¹ ; Gao, Haolei ¹ ; Liao, Chengshui ¹ ; Wang, Ruibiao ¹ ; Wen, Bo ¹ ; Luo, Weiyu ¹ ; Zhang, Wei ¹ ; Zhu, Chunling ¹ ; Wen, Yuliang ¹ ; Zhang, Longfei ¹ ; Liu, Xuehan ¹ ; Shen, Jiyuan ¹ ; Wu, Yundi ⁴ ; Wu, Xilong ⁴ ; Li, Hao ⁵ ; Hu, Jianhe ¹ ; Bai, Yueyu ¹ ; Zhang, Huihui ¹ ; Ding, Ke ¹ ; Wang, Lei ¹ ;

作者机构： 1.Henan Inst Sci & Technol, Coll Anim Sci & Vet Med, Xinxiang 453003, Peoples R China

2.Minist Educ, Key Lab Anim Pathogens & Biosafety, Zhengzhou 450000, Peoples R China

3.Henan Univ Sci & Technol, Lab Funct Microbiol & Anim Hlth, Luoyang 471023, Peoples R China

4.Hainan Univ, Sch Biomed Engn, State Key Lab Marine Resource Utilizat South China, Haikou 570228, Peoples R China

5.Chinese Acad Agr Sci, Inst Farmland Irrigat, Xinxiang 453003, Peoples R China

关键词： Antimicrobial peptide; Multiresistance; Piscidin 1; Bactericidal mechanism; Gram-negative bacteria

期刊名称：LWT-FOOD SCIENCE AND TECHNOLOGY （ 影响因子：6.6； 五年影响因子：6.9 ）

ISSN： 0023-6438

年卷期： 2025 年 215 卷

页码：

收录情况： SCI

摘要： The emergence and swift dissemination of multidrug-resistant (MDR) bacteria have posed a grave threat to public health. To counteract the escalating menace posed by MDR organisms, particularly mcr-1, blaNDM-1, or/ and tet (X3/X4)-positive Gram-negative pathogens, the identification of novel antimicrobial agents or treatment strategies is imperative. Antimicrobial peptides (AMPs) have emerged as potential antibiotic candidates. In this study, we discovered that piscidin 1, a cationic AMP derived from fish, exhibits potent antibacterial activity against MDR bacteria. Safety and stability assessments revealed that this peptide demonstrates robust resistance to various physiological conditions, possesses significant biofilm inhibition capabilities, but exhibits relatively low toxicity. Mechanistic experiments showed that piscidin 1 combats MDR pathogens through multiple channels, including permeabilization of inducing outer- and inner membrane, causing membrane depolarization, dissipating the proton motive force, inhibiting efflux pumps, reducing the intracellular ATP levels and causing the accumulation of NADH and ROS. Utilizing the mouse infection model, we demonstrate piscidin 1 displayed the excellent bactericidal activity against MDR pathogens in vitro. Collectively, these findings underscore the promising potential of piscidin 1 as a candidate for combating MDR pathogens.