Genome-wide identification of Shaker K+ channel family in Nicotiana tabacum and functional analysis of NtSKOR1B in response to salt stress
文献类型： 外文期刊
作者： Yuan, Guang 1 ; Nong, Tongjia 1 ; Hunpatin, Oluwaseyi Setonji 1 ; Shi, Chuhan 1 ; Su, Xiaoqing 1 ; Xu, Fangzheng 1 ; Wang, Yihui 4 ; Zhang, Zhaoting 5 ; Ning, Yang 1 ; Liu, Haobao 1 ; Wang, Qian 1 ;
作者机构： 1.Chinese Acad Agr Sci, Tobacco Res Inst, Qingdao, Peoples R China
2.Chinese Acad Agr Sci, Grad Sch, Beijing, Peoples R China
3.Qingdao Agr Univ, Coll Agr, Qingdao, Peoples R China
4.China Tobacco Shandong Ind Co LTD, Cigar Operat Ctr, Jinan, Peoples R China
5.Xuancheng City Xuanzhou Dist Tobacco Ind Dev Ctr, Xuancheng, Peoples R China
关键词： tobacco; Shaker K plus channel; SKOR1B; salt stress; K plus /Na plus ratio
期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：5.6； 五年影响因子：6.8 ）
ISSN： 1664-462X
年卷期： 2024 年 15 卷
页码：
收录情况： SCI
摘要： Soil salinization poses a mounting global ecological and environmental threat. The identification of genes responsible for negative regulation of salt tolerance and their utilization in crop improvement through gene editing technologies emerges as a swift strategy for the effective utilization of saline-alkali lands. One efficient mechanism of plant salt tolerance is maintaining the proper intracellular K+/Na+ ratio. The Shaker K+ channels play a crucial role in potassium absorption, transport, and intracellular potassium homeostasis in plant cells. Here, the study presents the first genome-wide identification of Shaker K+ channels in Nicotiana tabacum L., along with a detailed bioinformatic analysis of the 20 identified members. Transcriptome analysis revealed a significant up-regulation of NtSKOR1B, an outwardly-rectifying member predominantly expressed in the root tissue of tobacco seedlings, in response to salt stress. This finding was then confirmed by GUS staining of ProNtSKOR1B::GUS transgenic lines and RT-qPCR analysis. Subsequently, NtSKOR1B knockout mutants (ntskor1) were then generated and subjected to salt conditions. It was found that ntskor1 mutants exhibit enhanced salt tolerance, characterized by increased biomass, higher K+ content and elevated K+/Na+ ratios in both leaf and root tissues, compared to wild-type plants. These results indicate that NtSKOR1B knockout inhibits K+ efflux in root and leaf tissues of tobacco seedlings under salt stress, thereby maintaining higher K+/Na+ ratios within the cells. Thus, our study identifies NtSKOR1B as a negative regulator of salt tolerance in tobacco seedlings.
- 相关文献
作者其他论文 更多>>
-
Near complete genome assembly of Yadong trout (Salmo trutta)
作者：Li, Chen;Han, Shenglei;Li, Shuo;Liu, Kaiqiang;Liu, Yuyan;Wang, Hong-yan;Wang, Qian;Liu, Changlin;Shao, Changwei;Li, Chen;Li, Chen;Han, Shenglei;Li, Shuo;Liu, Kaiqiang;Liu, Yuyan;Wang, Hong-yan;Wang, Qian;Liu, Changlin;Shao, Changwei;Liu, Kaiqiang;Liu, Yuyan;Wang, Hong-yan;Wang, Qian;Shao, Changwei
关键词：
-
Shaker K+ channel NKT3A enhances potassium uptake and transport in tobacco (Nicotiana tabacum L.) seedlings under low potassium stress
作者：Xiang, Haiying;Xu, Li;Zhang, Jianduo;Mi, Qili;Gao, Qian;Yang, Wenwu;Huang, Haitao;Wang, Kunmiao;Zeng, Wanli;Yuan, Guang;Shi, Chuhan;Ning, Yang;Wang, Qian;Yuan, Guang;Shi, Chuhan
关键词：Tobacco; Potassium; Shaker K+ channel NKT3A; Uptake and transport
-
The full-length nsp2 replicase contributes to viral assembly in highly pathogenic PRRSV-2
作者：Bai, Yuan-Zhe;Wang, Shujie;Sun, Yue;Liu, Yong-Gang;Zhang, Hong-Liang;Wang, Qian;Huang, Rui;Rao, Cui-Hong;Xu, Shi-Jia;Tian, Zhi-Jun;An, Tong-Qing;Cai, Xue-Hui;Tang, Yan-Dong;Cai, Xue-Hui;Tang, Yan-Dong;Tang, Yan-Dong
关键词：arterivirus; PRRSV; nsp2; assembly; glycoprotein (GP); nucleocapsid (N) protein
-
Phenological Stage and Nitrogen Input Coordinately Regulate Bud Bank Dynamics and Shoot Allocation in an Alpine Clonal Perennial Grass
作者：He, Keyan;He, Lin;He, Lili;Dang, Haihong;Wei, Xiaoxing;He, Keyan;He, Lin;He, Lili;Dang, Haihong;Wei, Xiaoxing;Zhou, Qingping;Wang, Qian;Wang, Jiahao
关键词：belowground bud bank; nitrogen addition; phenological stage; shoot allocation; Qinghai-Tibet Plateau
-
Effects of Double-Stranded RNA Degrading Nucleases on RNAi Efficiency in Beet Moth Spodoptera exigua (Lepidoptera: Noctuidae)
作者：Wang, Guandi;Wang, Qian;Liu, Wenrui;Wen, Jingxin;Yang, Yubo;Niu, Zhilong;Guo, Wei;Zhao, Dan;Guo, Wei
关键词：RNA interference; dsRNA stability; dsRNase;
Spodoptera exigua ; nanomaterial-mediated RNAi -
The knockout of tobacco NtCBL10 inhibits leaf Cl- accumulation and leads to light-dependent necrosis & light-independent chlorosis under salt stress
作者：Mao, Jingjing;Yuan, Guang;Liu, Haobao;Ning, Yang;Wang, Qian;Mao, Jingjing;Yuan, Guang;Mao, Jingjing;Visser, Richard G. F.;Bai, Yuling;van der Linden, C. Gerard;Mao, Jingjing;Mao, Jingjing;Xu, Gang;Xue, Lin;Mao, Dongping
关键词：CBL10; Salt tolerance; Necrosis; Chlorosis; Light
-
Characterization of aroma profile and microbial community of cigar tobacco leaves from different aging periods and varieties and their correlations analysis
作者：Liu, Jian;Zhao, Junwei;Li, Xiang;Xiao, Yansong;Liu, Jian;He, Zelin;Lyu, Can;Ning, Yang;Lin, Qing;Wang, Xueying
关键词：Cigar; Microbial community; Aroma; Aging period; Variety
