您好，欢迎访问中国农业科学院 机构知识库！

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Two aquaporins, PIP1;1 and PIP2;1, mediate the uptake of neonicotinoid pesticides in plants

文献类型： 外文期刊

作者： Wan, Qun 1 ; Li, Yixin 2 ; Cheng, Jinjin 1 ; Wang, Ya 1 ; Ge, Jing 1 ; Liu, Tingli 3 ; Ma, Liya 1 ; Li, Yong 1 ; Liu, Jianan 2 ; Zhou, Chunli 2 ; Li, Haocong 2 ; Sun, Xing 1 ; Chen, Xiaolong 1 ; Li, Qing X. 4 ; Yu, Xiangyang 1 ;

作者机构： 1.State Key Lab Breeding Base, Key Lab Food Qual & Safety Jiangsu Prov, Nanjing 210014, Peoples R China

2.Jiangsu Acad Agr Sci, Inst Food Safety & Nutr, 50 Zhongling St, Nanjing 210014, Peoples R China

3.Nanjing Xiaozhuang Univ, Jiangsu Prov Key Construct Lab Special Biomass Res, 3601 Hongjin Ave, Nanjing 211171, Peoples R China

4.Univ Hawaii Manoa, Dept Mol Biosci & Bioengn, Honolulu, HI 96822 USA

关键词： neonicotinoid; aquaporin; Brassica rapa; plasma membrane; uptake; systemic pesticide

期刊名称：PLANT COMMUNICATIONS （ 影响因子：10.5； 五年影响因子：10.5 ）

ISSN： 2590-3462

年卷期： 2024 年 5 卷 5 期

页码：

收录情况： SCI

摘要： Neonicotinoids (NEOs), a large class of organic compounds, are a type of commonly used pesticide for crop protection. Their uptake and accumulation in plants are prerequisites for their intra- and intercellular movements, transformation, and function. Understanding the molecular mechanisms that underpin NEO uptake by plants is crucial for effective application, which remains elusive. Here, we demonstrate that NEOs enter plant cells primarily through the transmembrane symplastic pathway and accumulate mainly in the cytosol. Two plasma membrane intrinsic proteins discovered in Brassica rapa , BraPIP1;1 and BraPIP2;1, were found to encode aquaporins (AQPs) that are highly permeable to NEOs in different plant species and facilitate NEO subcellular diffusion and accumulation. Their conserved transport function was further demonstrated in Xenopus laevis oocyte and yeast assays. BraPIP1 ; 1 and BraPIP2 ; 1 gene knockouts and interaction assays suggested that their proteins can form functional heterotetramers. Assessment of the potential of mean force indicated a negative correlation between NEO uptake and the energy barrier of BraPIP1;1 channels. This study shows that AQPs transport organic compounds with greater osmolarity than previously thought, providing new insight into the molecular mechanisms of organic compound uptake and facilitating innovations in systemic pesticides.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台