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CRISPR/Cas9介导的多基因编辑系统在大豆中的应用

文献类型: 中文期刊

作者: 关贝贝 1 ; 王燕娟 1 ; 陈海峰 2 ; 陈水莲 2 ; 沙爱华 1 ; 曹东 2 ;

作者机构: 1.长江大学农学院

2.中国农业科学院油料作物研究所

关键词: 大豆;CRISPR/Cas9;GmAS1/2;GmSHP1/2;GmSTK;多基因编辑

期刊名称: 中国油料作物学报

ISSN:

年卷期: 2021 年 001 期

页码: 149-160

收录情况: 北大核心 ; CSCD

摘要: 自CRISPR/Cas9(clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9)基因组编辑技术发现以来,迅速在作物中得到广泛应用。但是,CRISPR/Cas9多基因编辑系统在大豆中的研究尚待开发。本文利用CRISPR/Cas9介导的多基因编辑系统,分别构建了两个载体,一个载体含6个靶点,编辑7个大豆基因(4个Glycine max ASYMMETRIC LEAVES1(GmAS1)同源基因和3个GmAS2同源基因),另一个载体含8个靶点,编辑11个G. max AGAMOUS家族同源基因(4个GmAG同源基因,2个G. max SEEDSTICK(GmSTK)同源基因和5个G. max SHATTERPROOF1(GmSHP1/2)同源基因)。大豆遗传转化后,经表型鉴定和靶点检测发现,CRISPR/Cas9介导的多基因编辑系统在大豆中成功实现了多基因编辑。当3个GmAS1同源基因和3个GmAS2同源基因同时突变时,导致大豆叶片向远轴面弯曲、皱缩且叶柄变短的表型。当2个GmSHP1同源基因和2个GmSTK同源基因同时突变时,导致豆荚停止发育的不育表型。

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