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利用小RNA深度测序快速鉴定草莓病毒

文献类型: 中文期刊

作者: 何成勇 1 ; 高德航 1 ; 范灵姣 1 ; 邢飞 1 ; 李世访 1 ; 王红清 1 ;

作者机构: 1.中国农业大学园艺学院;中国农业科学院植物保护研究所/植物病虫害生物学国家重点实验室

关键词: 草莓;sRNA测序;草莓白化病毒;草莓毛形病毒;RT-PCR

期刊名称: 中国农业大学学报

ISSN: 1007-4333

年卷期: 2021 年 008 期

页码: 54-60

收录情况: 北大核心 ; CSCD

摘要: 病毒侵染直接影响草莓的生长发育及果实品质,快速检测及鉴定病毒是病毒防治的前提。本研究以‘丰香’草莓和‘哈尼’草莓为试材,利用小RNA(sRNA)测序结合RT-PCR技术对2种栽培草莓品种中存在的病毒进行了研究。对sRNA测序结果进行组装和注释,结果表明:在混合样品中共有242个contigs比对到5种草莓病毒,分别为草莓白化病毒(strawberry pallidosis associated virus,SPaV)、草莓镶脉病毒(strawberry vein banding virus,SVBV)、草莓斑驳病毒(strawberry mottle virus,SMoV)、草莓毛形病毒3(strawberry crinivirus 3,SCrV 3)和草莓毛形病毒4(strawberry crinivirus 4,SCrV 4)。利用RT-PCR技术对sRNA测序结果进行验证,结果表明:SMoV、SVBV和SCrV 3在‘丰香’草莓和‘哈尼’草莓中均检测到,而SPaV和SCrV 4只在‘哈尼’草莓样品中检测到。本研究利用sRNA测序技术鉴定了2个草莓品种中存在的病毒,首次在我国生产的同一个草莓品种中检测到5种病毒,为草莓病毒的检测和防控提供了新的思路。

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