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敲除TMS5基因获得温敏不育粳稻新材料

文献类型: 中文期刊

作者: 杜茜 1 ; 费云燕 1 ; 王芳权 1 ; 许扬 1 ; 王军 1 ; 李文奇 1 ; 赵凌 1 ; 陈智慧 1 ; 梁国华 1 ; 周勇 1 ; 杨杰 1 ;

作者机构: 1.扬州大学江苏省粮食作物现代产业技术协同创新中心;江苏省农业科学院粮食作物研究所/国家水稻改良中心南京分中心/江苏省优质水稻工程技术研究中心

关键词: 粳稻;CRISPR/Cas9;温敏雄性不育系

期刊名称: 中国水稻科学

ISSN: 1001-7216

年卷期: 2019 年 05 期

页码: 429-435

收录情况: 北大核心 ; CSCD

摘要: 【目的】水稻温敏不育系的选育是两系杂交稻育种工作中的关键环节。TMS5是控制温敏不育的重要基因,在生产上也应用较广。为了加快水稻温敏不育系的选育进程,【方法】利用CRISPR/Cas9技术,在武运粳7号背景下对TMS5基因进行编辑。【结果】通过测序从22株T_0代中筛选获得6株纯合的突变体,分析发现6个纯合的突变体产生了相同的遗传变异,都在TMS5基因第2外显子44 bp和45 bp之间插入碱基"A",导致翻译提前终止。通过细胞学观察和人工辅助授粉证实获得的株系花粉败育而雌配子正常发育。【结论】这些结果表明对温敏不育基因TMS5基因进行编辑可以快速获得粳型温敏雄性不育系。

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