文献类型: 中文期刊
作者: 张文宣 1 ; 梁晓梅 1 ; 戴成 1 ; 文静 1 ; 易斌 1 ; 涂金星 1 ; 沈金雄 1 ; 傅廷栋 1 ; 马朝芝 1 ;
作者机构: 1.华中农业大学作物遗传改良全国重点实验室/国家油菜工程技术研究中心/洪山实验室
关键词: 甘蓝型油菜;BnaMPK6;CRISPR/Cas9;盐胁迫
期刊名称: 作物学报
ISSN: 0496-3490
年卷期: 2023 年 49 卷 002 期
页码: 321-331
收录情况: 北大核心 ; CSCD
摘要: 甘蓝型油菜是我国一种重要的油料作物.MPK6是一种丝裂原活化蛋白激酶,MAPK级联途径可以被各种胁迫激活,进而调节植物对胁迫的响应,在植物响应非生物胁迫过程中发挥着重要的作用,但在甘蓝型油菜盐胁迫响应过程中的功能还尚不清楚.基因结构和蛋白序列分析表明,MAPK6在十字花科芸薹属植物种间分化相对保守,基因结构相似,蛋白之间均有相同的STKc_TEY_MAPK结构域,与胁迫和生长发育相关.为探究其功能,本研究利用CRISPR/Cas9技术对BnaMPK6基因进行编辑,通过农杆菌介导的方法将BnaMPK6基因转入到甘蓝型油菜中,获得了BnaMPK6基因4个同源拷贝同时突变的材料:cr-bnampk6-13-1和cr-bnampk6-49-1.cr-bnampk6突变体表现出明显的盐敏感性:在100 mmol L-1和150 mmol L-1浓度的NaCl溶液处理下,功能缺失突变体长势明显缓慢,并且株高和鲜重显著低于野生型植株,但根长无明显差异.此外,在盐胁迫下,功能缺失突变体体内的活性氧和丙二醛含量的积累显著高于野生型对照,脯氨酸含量也明显高于野生型植株.本研究结果表明,BnaMPK6基因在盐水胁迫下正向调控植株的生长和发育,影响甘蓝型油菜的耐盐性.本研究不仅为BnaMPK6基因调控甘蓝型油菜盐胁迫的研究提供了理论基础,而且为甘蓝型油菜耐盐性遗传改良提供了一定的技术支持.
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