New Hydrolase from Aeromicrobium sp. HA for the Biodegradation of Zearalenone: Identification, Mechanism, and Application

文献类型： 外文期刊

第一作者： Hu, Junqiang

作者： Hu, Junqiang;Wang, Gang;Hou, Mingxuan;Du, Shilong;Han, Jun;Yu, Yangguang;Gao, Hongxia;He, Dan;Shi, Jianrong;Liu, Xin;Xu, Jianhong;Hu, Junqiang;Hong, Qing;Xu, Jianhong;Wang, Gang;Yu, Yangguang;Gao, Hongxia;Liu, Xin;Lee, Yin-Won;Mohamed, Sherif Ramzy;Dawood, Dawood H.

作者机构：

关键词： zearalenone; biodegradation; Aeromicrobium; hydrolase; estrogenic toxicity

期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：6.1； 五年影响因子：6.3 ）

ISSN： 0021-8561

年卷期： 2023 年

页码：

收录情况： SCI

摘要： Zearalenone (ZEN) is an estrogenic mycotoxin most frequently found in cereals that can cause reproductive disorders in livestock and pose a severe threat to animal husbandry. In this study, we isolated a ZEN-degrading Aeromicrobium strain from soil and found that ZenH, a hydrolase, is responsible for the hydrolysis of ZEN through comparative proteomics and biochemical studies. ZenH exhibited the highest similarity with lactone hydrolase ZHD607 from Phialophora americana at 21.52%. ZenH displayed maximal enzymatic activity at pH 7.0 and 55 degrees C with a Michaelis constant of 12.64 mu M. The catalytic triad of ZenH was identified as S117-D142-H292 by molecular docking and site-directed mutagenesis. ZenH catalyzed the hydrolysis of ZEN to a novel metabolite, (S,E)-4-hydroxy-2-(10-hydroxy-6-oxoundec-1-en-1-yl)-7-oxabicyclo[4.2.0]octa-1,3,5-trien-8-one, which exhibited significantly lower estrogenic toxicity than ZEN. This study illustrates a novel ZEN-degrading enzyme and reveals a new degradation product. Furthermore, the enzyme showed good potential for detoxifying ZEN during food processing.

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