Comparative Genomic Analysis of the Thiolase Family and Functional Characterization of the Acetyl-Coenzyme A Acyltransferase-1 Gene for Milk Biosynthesis and Production of Buffalo and Cattle
文献类型: 外文期刊
第一作者: Deng, Tingxian
作者: Deng, Tingxian;Wu, Jiyun;Wang, Xiaojie;Shaukat, Aftab;Zhou, Xiang;Zhou, Yang;Sun, Hui;Wei, Pengfei;Sun, Nan;Huang, Qianzhi;Xu, Linghua;Liu, Miaoyu;Lin, Yuxin;Yang, Liguo;Hua, Guohua;Deng, Tingxian;Wu, Jiyun;Sun, Hui;Hua, Guohua;Wu, Jiyun;Sun, Hui;Hua, Guohua;Abdel-Shafy, Hamdy;Shaukat, Aftab;Yang, Liguo;Hua, Guohua
作者机构:
关键词: ACAA1; cattle; buffalo; mammary epithelial cells; cell proliferation; milk production traits
期刊名称:JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY ( 影响因子:6.1; 五年影响因子:6.3 )
ISSN: 0021-8561
年卷期: 2023 年 71 卷 7 期
页码:
收录情况: SCI
摘要: Cattle and buffalo served as the first and second largest dairy animals, respectively, providing 96% milk products worldwide. Understanding the mechanisms underlying milk synthesis is critical to develop the technique to improve milk production. Thiolases, also known as acetyl-coenzyme A acetyltransferases (ACAT), are an enzyme family that plays vital roles in lipid metabolism, including ACAT1, ACAT2, ACAA1, ACAA2, and HADHB. Our present study showed that these five members were orthologous in six livestock species including buffalo and cattle. Transcriptomic data analyses derived from different lactations stages showed that ACAA1 displayed different expression patterns between buffalo and cattle. Immunohistochemistry staining revealed that ACAA1 were dominantly located in the mammary epithelial cells of these two dairy animals. Knockdown of ACAA1 inhibited mammary epithelial cell proliferation and triglyceride and beta-casein secretion by regulating related gene expressions in cattle and buffalo. In contrast, ACAA1 overexpression promoted cell proliferation and triglyceride secretion. Finally, three novel SNPs (g.-681A>T, g.-23117C>T, and g.-24348G>T) were detected and showed significant association with milk production traits of Mediterranean buffaloes. In addition, g.-681A>T mutation located in the promoter region changed transcriptional activity significantly. Our findings suggested that ACAA1 play a key role in regulating buffalo and cattle milk synthesis and provided basic information to further understand the dairy animal lactation physiology.
分类号:
- 相关文献
作者其他论文 更多>>
-
Comparative alternative polyadenylation profiles in differentiated adipocytes of subcutaneous and intramuscular fat tissue in cattle
作者:Meng, Xiangge;Hei, Yu;Zhou, Xiang;Li, Chengping;Zhou, Guoli;Zhou, Xiang;Zhou, Xiang;Zhou, Xiang
关键词:Alternative polyadenylation; Intramuscular adipocytes; Subcutaneous adipocytes; Differentiation; Cattle
-
Patterns and driving factors of soil organic carbon sequestration efficiency under various manure regimes across Chinese croplands
作者:Ren, Fengling;Zhang, Ruqiang;Zhang, Fusuo;Xu, Wen;Ren, Fengling;Sun, Nan;Xu, Minggang;Li, Yalin;Xu, Minggang
关键词:China 's cropland; Animal manure application; Soil organic carbon; Carbon sequestration efficiency; Random Forest
-
Encapsulation and delivery of phage as a novel method for gut flora manipulation in situ: A review
作者:Yang, Yufan;Zou, Geng;Zhou, Yang;Li, Jinquan;Yang, Yufan;Zou, Geng;Li, Jinquan;Du, Hu;Song, Zhiyong;Li, Hao;Tanf, Chen;Chen, Huanchun;Fischetti, Vincent A.;Li, Jinquan;Li, Jinquan
关键词:Gut flora regulation; Phage delivery; Encapsulation materials; Encapsulation carriers
-
One-Pot Assay for Rapid Detection of Benzimidazole Resistance in Venturia carpophila by Combining RPA and CRISPR/Cas12a
作者:Hu, Jia-Jie;Liu, Duo;Cai, Min-Zheng;Luo, Chao-Xi;Hu, Jia-Jie;Liu, Duo;Cai, Min-Zheng;Luo, Chao-Xi;Zhou, Yang;Yin, Wei-Xiao
关键词:fungicide resistance; one-pot detection; MBC fungicides; Venturia carpophila; RPA; CRISPR/Cas12a
-
Characterization and evolutionary analysis of phosphate starvation response genes in wheat and other major gramineous plants
作者:Sun, Renwei;Gong, Jie;Zhang, Shengquan;Ma, Jinxiu;Zhang, Fengting;Liu, Yongjie;Liao, Xiangzheng;Sun, Hui;Pang, Binshuang;Zhao, Changping;Gao, Shiqing;Sun, Renwei;Gong, Jie;Du, Yinke;Ma, Jinxiu;Liu, Yongjie;Pang, Binshuang;Zhao, Changping;Gao, Shiqing;Du, Yinke;Zheng, Wei;Sun, Renwei
关键词:Phosphate starvation; Phylogeny; Protein-protein interaction
-
Characterization of RING-type ubiquitin SINA E3 ligases and their responsive expression to salt and osmotic stresses in Brassica napus
作者:Fang, Fengyan;Zhou, Wenlong;Liu, Yanfeng;Song, Zhizhong;Zheng, Songfeng;Wang, Fei;Lu, Zeyu;Qi, Dazhuang;Li, Bei;Sun, Nan;Tang, Xiaoli;Zhang, Juan;Wang, Limin;Zhang, Hongxia;Fang, Fengyan;Zhou, Wenlong;Zheng, Songfeng;Wang, Fei;Lu, Zeyu;Qi, Dazhuang;Zhang, Juan;Wang, Limin;Zhang, Hongxia;Fang, Fengyan;Zhou, Wenlong;Liu, Yanfeng;Song, Zhizhong;Zheng, Songfeng;Wang, Fei;Lu, Zeyu;Qi, Dazhuang;Li, Bei;Sun, Nan;Tang, Xiaoli;Zhang, Juan;Wang, Limin;Zhang, Hongxia;Zhan, Renhui;Tang, Xiaoli;Wang, Limin;Zhang, Hongxia
关键词:Brassica napus; SINA; E3 ubiquitin ligase; Salt stress; Osmotic stress
-
Comparative analyses of copy number variations between swamp buffaloes and river buffaloes
作者:Yang, Lv;Han, Jiazheng;Li, Fan;Han, Xiaotao;Xia, Han;Hua, Guohua;Yang, Liguo;Zhou, Yang;Yang, Lv;Han, Jiazheng;Li, Fan;Han, Xiaotao;Xia, Han;Hua, Guohua;Yang, Liguo;Zhou, Yang;Deng, Tingxian;Quan, Fanfan;Hua, Guohua;Yang, Liguo;Zhou, Yang
关键词:CNV; F-ST; population structure analysis; river buffalo; swamp buffalo