Characterization of avain metapneumovirus subgroup C isolated from chickens in Beijing, China
文献类型: 外文期刊
第一作者: Wang, Jing
作者: Wang, Jing;Wei, Li;Yan, Xv;Zhu, Shanshan;Quan, Rong;Li, Zixuan;Wang, Dan;Jiang, Haijun;Song, Jiangwei;Hou, Lei;Liu, Jue;Hou, Lei;Liu, Jue
作者机构:
关键词: avian metapneumovirus subgroup C (aMPV; C); isolation and characterization; respiratory disease; nucleoprotein gene sequencing; pathogenicity
期刊名称:POULTRY SCIENCE ( 影响因子:4.014; 五年影响因子:4.192 )
ISSN: 0032-5791
年卷期: 2023 年 102 卷 1 期
页码:
收录情况: SCI
摘要: Avian metapneumovirus (aMPV) is an important causative agent that causes acute respiratory disease and egg-dropping in chickens and turkeys. Here, we characterized an aMPV subgroup C (aMPV/C) from 320-day-old broiler breeder chickens with severe respiratory diseases in Beijing, China, as evidenced by RT-PCR typing and confirmation of the nucleoprotein (N) gene sequence. The N gene sequence of the aMPV/ C strain (designated BJ17) exhibited no deletions or insertions and possessed 94.6% to 99.6% identity to those of published aMPV/C isolates. The phylogenetic tree of the nucleotide sequences constructed using the neighbor-joining clustering method showed that the BJ17 strain formed one cluster with other aMPV/C viruses and formed one subcluster with published Chi-nese aMPV/C isolates regardless of Muscovy duck or chicken origins. Comparative analysis of the N proteins showed that a unique amino acid residue D at position 110 might be associated with regional distribution due to its occurrence in all the Chinese aMPV/C isolates only. Strain BJ17 was successfully isolated by cultured Vero cell passage and further inoculated in 3-wk-old spe-cific-pathogen-free chickens for the examination of path-ogenicity. Animal experimental results showed that BJ17-inoculated chickens had severe respiratory diseases and inflammatory lesions, as demonstrated by patholog-ical changes and aMPV antigen in the nasal turbinate, tracheae, and lung tissues. These results enrich the avail-able information regarding the epidemiology and patho-genicity of aMPV/C in chickens, which may facilitate the development of effective measures against aMPV/C infection in China.
分类号:
- 相关文献
作者其他论文 更多>>
-
Environmental risk thresholds and prediction models of Cd in Chinese agricultural soils
作者:Yu, Lei;Chen, Shibao;Wang, Jing;Qin, Luyao;Sun, Xiaoyi;Wang, Meng;Zhang, Xing;Zhang, Xing;Chen, Shibao;Wang, Meng;Zhang, Xing
关键词:Soil Cd; Soil environmental risk threshold; Derivation methodology; Hormesis; SSD; Hc3
-
Mixed sowing of Feed rape and Vicia villosa can substitute nitrogen fertilizer to improve soil multifunctionality in the Hetao irrigation District
作者:Yu, Ru;Zhang, Hongyuan;Chang, Fangdi;Song, Jiashen;Wang, Jing;Wang, Xiquan;Li, Yuyi;Kan, Zhengrong;Zhao, Na;Li, Xiaohong;Ma, Jun
关键词:Green manure types; Nitrogen fertilizer; Soil multifunctionality; Yield
-
Integrated metabolomics and transcriptomics reveals difference involved in flavonoid and indole alkaloids biosynthesis in potato tuber flesh
作者:Wu, Juan;Ma, Yanhong;Zhang, Zhicheng;Zhang, Sheng;Wu, Xiaojuan;Wang, Peijie;Xie, Rui;Lu, Chunxing;Zhang, Zhicheng;Wang, Dan;Ma, Yanhong
关键词:Colored potato tuber flesh; HybridsF1; Flavonoid; Indole alkaloids; Correlation analysis
-
Facile preparation of biomimetic mineralized COFs based on magnetic silk fibroin and its effective extraction of sulforaphane from cruciferous vegetables
作者:Liu, Guangyang;Zhang, Xuan;Li, Lingyun;Chen, Ge;Lv, Jun;Xu, Xiaomin;Huang, Xiaodong;Xu, Donghui;Liu, Guangyang;Zhang, Xuan;Wang, Jian;Cao, Jiayong;Yin, Chen;Liu, Yuan;Zhang, Xuan;Xu, Donghui;Wang, Jing
关键词:Sulforaphane; Biomimetic mineralization; Silk fibroin; Covalent organic framework; Magnetic solid-phase extraction
-
Identification and functional prediction of CircRNAs of developing seeds in high oleic acid sunflower (Helianthus annuus L.)
作者:Zhou, Fei;Huang, Xutang;Zhou, Fei;Liu, Yan;Wang, Wenjun;Wu, Liren;Ma, Jun;Zhang, Shuquan;Wang, Jing;Yuan, Hongmei;Huang, Xutang;Feng, Fujuan
关键词:Sunflower; CircRNAs; Host genes; Seed development; Oil biosynthesis; High oleic acid
-
Yeast cell-wall polysaccharides improve immunity and attenuate inflammatory response via modulating gut microbiota in LPS-challenged laying hens
作者:Zhou, Jianmin;Fu, Yu;Qi, Guanghai;Zhang, Haijun;Wang, Jing;Wu, Shugeng;Dai, Jinjun;Wang, Jing
关键词:Yeast cell wall polysaccharides; Immunity; Inflammatory response; Gut microbiota; Laying hen
-
Creating burdock polysaccharide-oleanolic acid-ursolic acid nanoparticles to deliver enhanced anti-inflammatory effects: fabrication, structural characterization and property evaluation
作者:Zhu, Shanshan;Qiu, Zhichang;Qiao, Xuguang;Zhu, Wenqing;Zheng, Zhenjia;Waterhouse, Geoffrey I. N.;Zhao, Wenting;He, Qiuxia
关键词:Encapsulation; Structural features; Particle size; Zeta potential; Thermodynamic properties; In vivo verification