Development of a rapid and sensitive reverse transcription real-time quantitative PCR assay for detection and quantification of grass carp reovirus II
文献类型: 外文期刊
第一作者: Li, Jiahao
作者: Li, Jiahao;Wang, Yajun;Wang, Yingying;Li, Yingying;Shi, Cunbin;Mo, Xubing;Wang, Qing;Yin, Jiyuan;Wu, Huiliang;Xu, Wei;Wang, Hao;Bergmann, Sven M.;Wang, Qing;Yin, Jiyuan
作者机构:
关键词: GCRV-II; Quantitative analysis; RT-qPCR; Detection
期刊名称:JOURNAL OF VIROLOGICAL METHODS ( 影响因子:3.1; 五年影响因子:2.4 )
ISSN: 0166-0934
年卷期: 2023 年 312 卷
页码:
收录情况: SCI
摘要: Hemorrhagic disease of grass carp, which is induced by grass carp reovirus II (GCRV-II), leads to mass mortality in grass carp culture and causes enormous economic loss. However, there is currently no quantitative analysis method for the detection of GCRV-II, which is greatly restricted the etiological and epidemiological study of the disease. In this study a reverse transcription TaqMan PCR (RT-qPCR) assay was developed for the quantitative detection of GCRV-II. The probe and primers targeted location is the segment 6 (S6) region of the GCRV-II genome which is highly conserved. Standard curves were drawn and criteria were confirmed after the deter-mination of the optimum reaction conditions. The species-specific assay showed that the method is highly specific and has no cross reactions with other pathogens. The assay was sufficiently sensitive to detect as low as 10 copies of virus RNA. Moreover, the method has a very good repeatability for batches and inter-batches sample detection. Then the method was applied to detect the virus in tissue samples from clinically infected grass carp, compared with conventional RT-seminested PCR, the RT-qPCR represents a specific value for detection rate of positive samples. In summary, the RT-qPCR was applied and achieved high sensitivity and specificity for GCRV-II detection.
分类号:
- 相关文献
作者其他论文 更多>>
-
Systematic assessment of the antifungal mechanism of soil fumigant methyl isothiocyanate against Fusarium oxysporum
作者:Zhang, Daqi;Ren, Lirui;Wang, Qing;Li, Wenjing;Song, Zhaoxin;Fang, Wensheng;Yan, Dongdong;Li, Yuan;Wang, Qiuxia;Cao, Aocheng;He, Lin;Jin, Xi;Cao, Aocheng;Fang, Wensheng;Yan, Dongdong;Li, Yuan;Wang, Qiuxia;Cao, Aocheng;Cao, Aocheng
关键词:Methyl isothiocyanate; Antimicrobial mechanism; Transcriptome sequencing; Soil-borne fungi; Oxidative stress
-
The responsive mechanisms of DNA methylation and transcriptional regulation to acute hypoxia stress in HIF-1/VEGFA signal pathway of Japanese flounder (Paralichthys olivaceus)
作者:Liu, Binghua;Li, Guangling;Li, Xiaohui;Wang, Hao;Yang, Jun;Wen, Haishen;He, Feng;Liu, Binghua
关键词:DNA methylation; Transcriptional regulation; Binding site; Hypoxia stress; Signal pathway; Japanese flounder
-
The regulatory mechanisms of delayed senescence of nitric oxide treatment of hyacinth beans
作者:Han, Lichun;Ma, Lili;He, Xuelian;Bai, Chunmei;Wang, Hongwei;Wang, Qing;Zuo, Jinhua;Zheng, Yanyan;Han, Lichun;Wang, Zhengrong;He, Xuelian;Watkins, Christopher B.;Wang, Qing;Zuo, Jinhua;Zheng, Yanyan
关键词:Sodium Nitroprusside; Metabolomics; Transcriptomics; ATAC-seq; Flavonoids
-
Influence of linoleic acid on the immunodetection of shrimp (Litopenaeus vannamei) tropomyosin and the mechanism investigation via multi-spectroscopic and molecular modeling techniques
作者:Zhao, Jinlong;Wang, Hao;Zhang, Ziye;Lin, Hong;Li, Zhenxing;Zhao, Jinlong;Zhao, Jinlong;Liu, Yuhai;Xu, Lili;Sun, Lirui;Chen, Guanzhi;Li, Zhenxing
关键词:Shrimp(Litopenaeusvannamei) tropomyosin; Linoleic acid; Matrix effect; Immunodetection; Spectroscopic analysis; Molecular modeling
-
Comparative analysis of allergenicity and predicted linear epitopes in alpha and beta parvalbumin from turbot (Scophthalmus maximus)
作者:Li, Zhenxing;Huang, Yuhao;Li, Zhenxing;Wu, Yeting;Zhang, Ziye;Wang, Hao;Lin, Hong;Li, Yonghong;Pramod, Siddanakoppalu;Chen, Guanzhi;Zhu, Wenjia
关键词:parvalbumin; IgE binding; epitope; cross-reactivity; fish allergy
-
A new phragmalin-type limonoid from the roots of Trichilia sinensis
作者:Yang, Jin-Ling;Chen, Hui-Qin;Yang, Cheng-min;Wang, Hao;Cai, Cai-Hong;Yuan, Jing-Zhe;Mei, Wen-Li;Dai, Hao-Fu;Liu, Shou-Bai;Yang, Cheng-min
关键词:Trichilia sinensis; Meliaceae; Trichilinone A; AChE inhibitory activity
-
Effects of solid-state fermentation with Bacillus subtilis Y4 on the quality of Yibin Yacai
作者:Zou, Yuke;Zhong, Yixin;Zhou, Qian;Chen, Qian;Wu, Yanping;Zhong, Kai;Gao, Hong;Jia, Zhenchao;Xu, Wei;Wei, Shugu
关键词:Yibin Yacai; Bacillus subtilis Y4; Solid-state fermentation; Flavor -related metabolites; Microbial communities