Comparative analysis of allergenicity and predicted linear epitopes in alpha and beta parvalbumin from turbot (Scophthalmus maximus)

文献类型： 外文期刊

第一作者： Li, Zhenxing

作者： Li, Zhenxing;Huang, Yuhao;Li, Zhenxing;Wu, Yeting;Zhang, Ziye;Wang, Hao;Lin, Hong;Li, Yonghong;Pramod, Siddanakoppalu;Chen, Guanzhi;Zhu, Wenjia

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关键词： parvalbumin; IgE binding; epitope; cross-reactivity; fish allergy

期刊名称：JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE （ 影响因子：4.1； 五年影响因子：4.2 ）

ISSN： 0022-5142

年卷期： 2023 年 103 卷 5 期

页码：

收录情况： SCI

摘要： BACKGROUNDParvalbumin (PV) can be subdivided into two phylogenetic lineages, alpha PV and beta PV. The bony fish beta PV is considered a major fish allergen. However, there is no available report on the immunological property and epitope mapping of bony fish alpha PV. RESULTSTo characterize the allergenic property of bony fish alpha PV and investigate the difference in allergenic property of bony fish alpha PV and beta PV, turbot (Scophthalmus maximus) alpha PV and beta PV were identified by mass spectrometry and were expressed in Escherichia coli system in this study. Spectra analysis and three-dimensional (3D) modeling showed the similar structure between alpha PV and beta PV. However, alpha PV exhibited lower immunoglobulin E/immunoglobulin G (IgE/IgG) binding capacity than beta PV. Three identified beta PV epitopes possessed higher IgE reactivity and more hydrophobic residues than three identified alpha PV epitopes. In addition, less similarity in sequence homology of alpha PV epitopes was observed with allergen sequences in database. CONCLUSIONThese finding expanded information on fish PV epitopes and substantiated the difference in allergenicity and epitope mapping between fish alpha PV and beta PV, which will improve the epitope-based detection tools of PV and diagnostic of PV induced fish allergy. (c) 2023 Society of Chemical Industry.

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