Cloning and characterization of the EIN2-homology gene LeEIN2 from tomato
文献类型: 外文期刊
第一作者: Wang, Jia
作者: Wang, Jia;Chen, Guoping;Hu, Zongli;Chen, Xuqing
作者机构:
关键词: tomato; ethylene; Le EIN2; signal transduction
期刊名称:DNA SEQUENCE ( 影响因子:0.408; 五年影响因子:0.594 )
ISSN: 1042-5179
年卷期: 2007 年 18 卷 1 期
页码:
收录情况: SCI
摘要: A full-length cDNA, named LeEIN2 was cloned from tomato (Lycopersicon esculentum) by RT-PCR and RACE. Then the genomic DNA sequence of Le EIN2 was isolated by LA (Long and Accurate) PCR and Inverse PCR. This DNA sequence was 6838 bp including six introns. The Le EIN2 cDNA was 4343 bp and contained a 3951 bp open reading frame, encoding a 142.63 kDa protein of 1316 amino acids. Comparison of this protein sequence with that of Arabidopsis and Petunia showed that the amino acid homology was 66 and 90%, respectively. Northern blotting analysis indicated that the expression level of the LeEIN2 in young leaves was higher than in mature leaves and senescent leaves. During the development of fruits, the expression of the Le EIN2 was detected before mature green stage and got up to maximum at mature green and breaker stages, then reduced rapidly after breaker stage. The expression level of LeEIN2 in wild type tomato fruit at mature green stage did not distinctively change when treated with exogenous ethylene.
分类号:
- 相关文献
作者其他论文 更多>>
-
Ball milling-assisted preparation of sludge biochar as a novel periodate activator for nonradical degradation of sulfamethoxazole: Insight into the mechanism of enhanced electron transfer
作者:He, Liuyang;Yang, Shangding;Yang, Lie;Shen, Shitai;Li, Yulong;Kong, Dejin;Wu, Li;Zhang, Zulin;Chen, Zhuqi;Wang, Jia;Yang, Shengmao;Zhang, Zulin
关键词:Periodate; Magnetic sludge biochar; Ball milling; Electron transfer; Nonradical degradation
-
A tomato HD-zip I transcription factor, VAHOX1, acts as a negative regulator of fruit ripening
作者:Li, Fenfen;Fu, Mengjie;Zhou, Shengen;Xie, Qiaoli;Chen, Guoping;Hu, Zongli;Chen, Xuqing
关键词:
-
Suppression of a hexokinase gene SlHXK1 in tomato affects fruit setting and seed quality
作者:Li, Jing;Liu, Yu;Cao, Lili;Xie, Qiaoli;Chen, Guoping;Hu, Zongli;Chen, Xuqing;Zhang, Jianling;Hu, Zongli;Chen, Xuqing
关键词:Abscission zone; Hexokinase; Pollen; Seed; Starch; Tomato
-
Overexpression of SlPRE3 alters the plant morphologies in Solanum lycopersicum
作者:Guo, Pengyu;Yang, Qingling;Wang, Yunshu;Yang, Zhijie;Xie, Qiaoli;Chen, Guoping;Hu, Zongli;Chen, Xuqing
关键词:Cell development; Plant morphology; RNA-seq; SlPRE3; Solanum lycopersicum
-
Frontiers and advances in N-heterocycle compounds as corrosion inhibitors in acid medium: Recent advances
作者:Wang, Jia;An, Lu;Wang, Jun;Gu, Jie;Wang, Xiaojuan;Gu, Jie;Wang, Xiaojuan;Sun, Jian
关键词:Metal corrosion; Nitrogen-containing heterocyclic compounds; Acid medium; Corrosion inhibitors
-
Conserved Domains in Variable Surface Lipoproteins A-G of Mycoplasma hyorhinis May Serve as Probable Multi-Epitope Candidate Vaccine: Computational Reverse Vaccinology Approach
作者:Zubair, Muhammad;Wang, Jia;Yu, Yanfei;Shao, Guoqing;Feng, Zhixin;Xiong, Qiyan;Zubair, Muhammad;Wang, Jia;Yu, Yanfei;Shao, Guoqing;Feng, Zhixin;Xiong, Qiyan;Yu, Yanfei;Shao, Guoqing;Yu, Yanfei;Xiong, Qiyan;Rasheed, Muhammad Asif;Faisal, Muhammad;Dawood, Ali Sobhy;Dawood, Ali Sobhy;Ashraf, Muhammad;Xiong, Qiyan
关键词:conserved domains; immunoinformatics; multiepitope-based vaccine; M. hyorhinis; reverse vaccinology; variable surface lipoproteins
-
Transcriptome profiling of genes regulated by phosphate-solubilizing bacteria Bacillus megaterium P68 in potato (Solanum tuberosum L.)
作者:Lin, Lizhen;Ren, Zongling;Cai, Jianying;Zhao, Lanfeng;Cai, Yanfei;Li, Chengchen;Li, Xiaobo;Xiong, Xingyao;Qin, Yuzhi;Wang, Ruilong;Wang, Jia
关键词:Bacillus megaterium; transcriptome; potato; commercial tubers yield; phosphate solubilizing bacteria