Overexpression of SlPRE3 alters the plant morphologies in Solanum lycopersicum
文献类型: 外文期刊
第一作者: Guo, Pengyu
作者: Guo, Pengyu;Yang, Qingling;Wang, Yunshu;Yang, Zhijie;Xie, Qiaoli;Chen, Guoping;Hu, Zongli;Chen, Xuqing
作者机构:
关键词: Cell development; Plant morphology; RNA-seq; SlPRE3; Solanum lycopersicum
期刊名称:PLANT CELL REPORTS ( 影响因子:6.2; 五年影响因子:5.9 )
ISSN: 0721-7714
年卷期: 2023 年
页码:
收录情况: SCI
摘要: Basic helix-loop-helix (bHLH) transcription factors play crucial roles as regulators in plant growth and development. In this study, we isolated and characterized SlPRE3, an atypical bHLH transcription factor gene. SlPRE3 exhibited predominant expression in the root and moderate expression in the senescent leaves. Comparative analysis with the wild type revealed significant differences in plant morphology in the 35S:SlPRE3 lines. These differences included increased internode length, rolling leaves with reduced chlorophyll accumulation, and elongated yet fewer adventitious roots. Additionally, 35S:SlPRE3 lines displayed elevated levels of GA(3) (gibberellin A3) and reduced starch accumulation. Furthermore, utilizing the Y2H (Yeast two-hybrid) and the BiFC (Bimolecular Fluorescent Complimentary) techniques, we identified physical interactions between SlPRE3 and SlAIF1 (ATBS1-interacting factor 1)/SlAIF2 (ATBS1-interacting factor 2)/SlPAR1 (PHYTOCHROME RAPIDLY REGULATED 1)/SlIBH1 (ILI1-binding bHLH 1). RNA-seq analysis of root tissues revealed significant alterations in transcript levels of genes involved in gibberellin metabolism and signal transduction, cell expansion, and root development. In summary, our study sheds light on the crucial regulatory role of SlPRE3 in determining plant morphology and root development.
分类号:
- 相关文献
作者其他论文 更多>>
-
A tomato HD-zip I transcription factor, VAHOX1, acts as a negative regulator of fruit ripening
作者:Li, Fenfen;Fu, Mengjie;Zhou, Shengen;Xie, Qiaoli;Chen, Guoping;Hu, Zongli;Chen, Xuqing
关键词:
-
Suppression of a hexokinase gene SlHXK1 in tomato affects fruit setting and seed quality
作者:Li, Jing;Liu, Yu;Cao, Lili;Xie, Qiaoli;Chen, Guoping;Hu, Zongli;Chen, Xuqing;Zhang, Jianling;Hu, Zongli;Chen, Xuqing
关键词:Abscission zone; Hexokinase; Pollen; Seed; Starch; Tomato
-
SlERF.J2 reduces chlorophyll accumulation and inhibits chloroplast biogenesis and development in tomato leaves
作者:Chen, Yanan;Cai, Xi;Tang, Boyan;Xie, Qiaoli;Chen, Guoping;Hu, Zongli;Chen, Xuqing;Chen, Xuqing;Hu, Zongli
关键词:Chloroplast development; Chlorophyll; SlERF; J2; (COP1); Nr mutants; Tomato; CONSTITUTIVE PHOTOMORPHOGENIC 1
-
A Modified Method for Transient Transformation via Pollen Magnetofection in Lilium Germplasm
作者:Zhang, Mingfang;Xue, Jing;Du, Yunpeng;Chen, Xuqing;Yang, Fengping;Zhang, Xiuhai;Ma, Xu;Jin, Ge;Han, Dongyang;Zhao, Chunli
关键词:Lilium regale; genetic transformation system; pollen magnetofection; PBI121 and pYBA1132 transformation
-
Characterization of an Endophytic Antagonistic Bacterial Strain Bacillus halotolerans LBG-1-13 with Multiple Plant Growth-Promoting Traits, Stress Tolerance, and Its Effects on Lily Growth
作者:Gao, Jun-lian;Sun, Yu-chen;Xue, Jing;Du, Yunpeng;Chen, Xuqing;Zhang, Xiuhai;Khan, Mohammad Sayyar;Yang, Chunhong;Chebotar, Vladimir K.;Tikunov, Vladimir S.;Rubanov, Ivan N.
关键词:
-
Comparative Transcriptome and Interaction Protein Analysis Reveals the Mechanism of IbMPK3-Overexpressing Transgenic Sweet Potato Response to Low-Temperature Stress
作者:Jin, Rong;Guo, Pengyu;Liu, Ming;Zhao, Peng;Zhang, Qiangqiang;Zhu, Xiaoya;Wang, Jing;Zhang, Aijun;Cao, Qinghe;Tang, Zhonghou;Yu, Tao;Pan, Jiaquan
关键词:IbMPK3-overexpressing transgenic sweet potato plants; low-temperature stress; transcriptome analysis; protein interaction
-
Transcription and Metabolism Pathways of Anthocyanin in Purple Shamrock (Oxalis triangularis A.St.-Hil.)
作者:Luo, Baobing;Chen, Liujun;Chen, Guoping;Wang, Yunshu;Xie, Qiaoli;Hu, Zongli;Chen, Xuqing
关键词:anthocyanin biosynthesis; Oxalis triangularis; UPLC-ESI-MS; MS; RNA-seq; stress resistance