Comparative analysis of pre- and post-parasitic transcriptomes and mining pioneer effectors of Heterodera avenae

文献类型： 外文期刊

第一作者： Yang, Dan

作者： Yang, Dan;Chen, Changlong;Liu, Qian;Jian, Heng;Chen, Changlong

作者机构：

关键词： Heterodera avenae;Pre-parasitic and post-parasitic stage;Transcriptome sequencing;Effectors;Comparative analysis

期刊名称：CELL AND BIOSCIENCE （ 影响因子：7.133； 五年影响因子：6.302 ）

ISSN： 2045-3701

年卷期： 2017 年 7 卷

页码：

收录情况： SCI

摘要： Background: The cereal cyst nematode (CCN, Heterodera avenae) is a devastating pathogen of wheat and barley crops in many countries. We aimed to prioritize genetic and molecular targets for H. avenae control via the powerful and integrative bioinformatics platform. Results: Here, we sequenced mRNA isolated from Chinese H. avenae at pre-parasitic (consisting of egg, J1 and hatched-J2) stages and post-parasitic (consisting of parasitic-J2, J3, J4 and adults) stages. Total 1,066,719 reads of whole life cycle transcriptomes were assembled into 10,811 contigs with N50 length of 1754 bp and 71,401 singletons. Comparative analyses of orthologous among H. avenae and 7 other nematodes with various life-styles revealed the significance and peculiarity of neurological system for sedentary phytonematode. KEGG pathway enrichment demonstrated active crosstalk events of nervous system at pre-parasitic stages, and 6 FMRFamide-like neuropeptides were verified to display an expression peak at the hatched-J2 stage in H. avenae. Furthermore, multiple approaches were undertaken to mine putative effectors and parasitism-specific genes. Notably, H. avenae might represent the first phytonematode reported to possess the pioneer effectors with RxLR motif and potential effectors with homologies to Ant-5/Ant-34. Conclusion: Our work provides valuable resources for in-depth understanding the parasitism and pathogenicity of H. avenae, as well as developing new targets-oriented strategies on effective managements.

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