Suppressive Effects of Barley beta-Glucans with Different Molecular Weight on 3T3-L1 Adipocyte Differentiation

文献类型： 外文期刊

第一作者： Zhu, Yingying

作者： Zhu, Yingying;Yao, Yang;Gao, Yue;Hu, Yibo;Shi, Zhenxing;Ren, Guixing;Zhu, Yingying;Gao, Yue

作者机构：

关键词： barley beta-glucans;3T3-L1 cells;PPAR gamma;C/EBP alpha

期刊名称：JOURNAL OF FOOD SCIENCE （ 影响因子：3.167； 五年影响因子：3.376 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： In this study, 2 beta-glucans with different molecular weight were prepared and purified from hull-less barley bran. The aim was to evaluate their effects on the differentiation of 3T3-L1 pre-adipocytes. Results showed that barley beta-glucans inhibited the differentiation of 3T3-L1 pre-adipocytes induced by differentiation medium in a dose-dependent manner, the suppressive effect of high-molecular-weight barley beta-glucans (552 kDa, BGH) was stronger (P < 0.05) than that of low-molecular-weight barley beta-glucan (32 kDa, BGL), evidenced by the significantly decrease (P < 0.05) of Oil-red O staining and intracellular triglyceride content in the mature adipocytes. Besides, gene expression analysis and Western Blot analysis revealed that both BGH and BGL inhibited the mRNA and protein levels of adipogenesis related transcription factors peroxisome proliferator-activated receptor. (PPAR.) and CCAAT-enhancer-binding protein a (C/EBP alpha) which are principal regulators of adipogenesis. Furthermore, the mRNA and protein expression levels of PPAR. target genes in adipose tissue including adipocyte fatty acid binding protein (ap2), lipoprotein lipase (LPL), uncoupling protein-2 (UCP-2), and glucose-transporter 4 (Glut4) in 3T3-L1 cells was also markedly downregulated (P < 0.05). These findings were anticipated to help develop barley beta-glucans based functional food for the management of obesity.

分类号： TS2

  • 相关文献

[1]Isoflavones in Chickpeas Inhibit Adipocyte Differentiation and Prevent Insulin Resistance in 3T3-L1 Cells. Yao, Yang,Ren, Guixing,Gao, Yue,Zhu, Yinging.

[2]Postnatal expression pattern of PPAR gamma gene in pigs. Jiang, Junfang,Jiang, Yongqing,Zhu, Zhigang,Xu, Zirong. 2009

[3]Differential gene expression of bovine intramuscular and subcutaneous preadipocytes during differentiation. Liu, Xiaomu,Song, Enliang,Tan, Xiuwen,Liu, Guifen,Cheng, Haijian,Wan, Fachun,Liu, Xiaomu,Song, Enliang,Tan, Xiuwen,Liu, Guifen,Cheng, Haijian,Wan, Fachun,Zang, Kun,Ling, Zongshuai. 2012

[4]Mechanisms involved in ceramide-induced cell cycle arrest in human hepatocarcinoma cells. Wang, Jing,Lv, Xiao-Wen,Shi, Jie-Ping,Hu, Xiao-Song. 2007

[5]Troglitazone Induced Apoptosis via PPAR gamma Activated POX-induced ROS Formation in HT29 Cells. Wang Jing,Du YuGuo,Lv XiaoWen,Shi JiePing,Hu XiaoSong. 2011

[6]Identification and characterization of transcript variants of chicken peroxisome proliferator-activated receptor gamma. Duan, Kui,Sun, Yingning,Zhang, Xiaofei,Zhang, Wenjian,Zhang, Jiyang,Wang, Guihua,Wang, Shouzhi,Leng, Li,Li, Hui,Wang, Ning,Duan, Kui,Sun, Yingning,Zhang, Xiaofei,Zhang, Tianmu,Zhang, Wenjian,Zhang, Jiyang,Wang, Guihua,Wang, Shouzhi,Leng, Li,Li, Hui,Wang, Ning,Sun, Yingning.

[7]A-type ECG and EGCG dimers disturb the structure of 3T3-L1 cell membrane and strongly inhibit its differentiation by targeting peroxisome proliferator-activated receptor gamma with miR-27 involved mechanism. Zhu, Wei,Zou, Bo,Nie, Rongzu,Zhang, Ying,Li, Chun-mei,Zou, Bo,Li, Chun-mei.

[8]Anti-adipogenic Effects of Polyphenol Extracts of Areca Flower Tea on 3T3-L1 Preadipocytes. Song, Fei,Tang, MinMin,Shen, XiaoJun,Wang, Hui,Chen, Hua,Zhao, SongLin,Wu, QiuSheng.

[9]PPAR gamma is regulated by miR-27b-3p negatively and plays an important role in porcine oocyte maturation. Song, Chunlei,Liang, Xiaojuan,Tao, Cong,Li, Kui,Wang, Yanfang,Yao, Jing,Cao, Chunwei,Huang, Jiaojiao,Han, Ziqiang,Zhang, Yang,Qin, Guosong,Zhao, Jianguo,Yao, Jing,Cao, Chunwei,Huang, Jiaojiao,Zhang, Yang,Qin, Guosong,Zhao, Jianguo,Han, Ziqiang,Li, Chengbo,Yang, Haoran.

作者其他论文 更多>>

微信小程序