Genetic Polymorphism and mRNA Expression Studies Reveal IL6R and LEPR Gene Associations with Reproductive Traits in Chinese Holsteins
文献类型: 外文期刊
第一作者: Zhang, Hailiang
作者: Zhang, Hailiang;Sammad, Abdul;Shi, Rui;Dong, Yixin;Wang, Yachun;Zhao, Shanjiang;Liu, Lin;Guo, Gang;Xu, Qing;Liu, Aoxing
作者机构:
关键词: Holstein cattle; fertility; gene expression; ovarian follicle; haplotype
期刊名称:AGRICULTURE-BASEL ( 影响因子:3.6; 五年影响因子:3.6 )
ISSN:
年卷期: 2023 年 13 卷 2 期
页码:
收录情况: SCI
摘要: Genetic selection of milk yield traits alters the energy distribution of high producing cows, resulting in gene-induced negative energy balance, and consequently, poor body condition scores and reduced reproductive performances. Here, we investigated two metabolic-syndrome pathway genes, IL6R (Interleukin 6 receptor) and LEPR (Leptin receptor), for their polymorphism effects on reproductive performance in dairy cows, by applying polymorphism association analyses in 1588 Chinese Holstein cows (at population level) and gene expression analyses in granulosa cells isolated from eight cows (at cell level). Among the six single nucleotide polymorphisms we examined (two SNPs for IL6R and four SNPs for LEPR), five were significantly associated with at least one reproductive trait, including female fertility traits covering both the ability to recycle after calving and the ability to conceive and keep pregnancy when inseminated properly, as well as calving traits. Notably, the identified variant SNP g.80143337A/C in LEPR is a missense variant. The role of IL6R and LEPR in cattle reproduction were further confirmed by observed differences in relative gene expression levels amongst granulosa cells with different developmental stages. Collectively, the functional validation of IL6R and LEPR performed in this study improved our understanding of cattle reproduction while providing important molecular markers for genetic selection of reproductive traits in high-yielding dairy cattle.
分类号:
- 相关文献
作者其他论文 更多>>
-
A versatile AIE probe with two cross-talk-free emissions for dual detection of SO2 and viscosity and its application in food and biological imaging
作者:Zhang, Di;Li, Man;Liu, Lin;Duan, Ran;Xue, Nana;Chen, He;Shang, Lijun;Wang, Tieliang;Wu, Xujin;Zhang, Junfeng;Liu, Lin;Wang, Min
关键词:Sulfur dioxide; Viscosity; Cross-talk-free emissions; AIE; Mitochondria targeting; Food samples
-
Characterization and potential function of 7-dehydrocholesterol reductase (dhcr7) and lathosterol 5-desaturase (sc5d) in Cynoglossus semilaevis sexual size dimorphism
作者:Wang, Jialin;Shi, Rui;Yang, Qian;Chen, Zhangfan;Wang, Jiacheng;Gong, Zhihong;Chen, Songlin;Wang, Na;Wang, Jialin;Shi, Rui;Yang, Qian;Wang, Jiacheng;Gong, Zhihong;Chen, Zhangfan;Chen, Songlin;Wang, Na;Chen, Zhangfan;Chen, Songlin;Wang, Na
关键词:Chinese tongue sole ( Cynoglossus semilaevis ); 7-dehydrocholesterol reductase ( dhcr7 ); lathosterol 5-desaturase ( sc5d ); Promoter activity; siRNA; Protein subcellular localization
-
A mitochondria-targeted fluorescent probe for reversible recognition of sulfur dioxide/formaldehyde and its application in cell imaging
作者:Liu, Lin;Shi, Jiacheng;Guo, Fengqi;Liu, Lin;Zhang, Di;Li, Man;Shi, Jiacheng;Guo, Jie;Wang, Tieliang
关键词:Sulfur dioxide; Fluorescent probe; Reversible; Mitochondria; Formaldehyde
-
Isolation and Characterization of SPOTTED LEAF42 Encoding a Porphobilinogen Deaminase in Rice
作者:Liu, Lin;Wang, Yunpeng;Tian, Yunlu;Song, Shuang;Wu, Zewan;Ding, Xin;Zheng, Hai;Huang, Yunshuai;Liu, Shijia;Dong, Xiaoou;Wan, Jianmin;Liu, Linglong;Wan, Jianmin
关键词:chlorophyll biosynthesis; chloroplast; rice (Oryza sativa L; ); RNA editing; spotted leaf; porphobilinogen deaminase (PBGD)
-
Sirtuin 3 regulation: a target to alleviate beta-hydroxybutyric acid-induced mitochondrial dysfunction in bovine granulosa cells
作者:Zhao, Shanjiang;Gong, Jianfei;Wang, Yi;Heng, Nuo;Wang, Huan;Hu, Zhihui;Wang, Haoyu;Zhang, Haobo;Zhu, Huabin
关键词:BHBA; Dairy cows; Granulosa cells; Ketosis; Mitochondrial function; Sirt3
-
Transcriptome profiling of bovine endometrial epithelial cells induced by lipopolysaccharides in vitro
作者:Zhou, GuangWei;Shen, PuXiu;Sun, Yu;Zhang, Xing;Yan, ChenBo;Yu, JingCheng;Liu, Fang;Yang, DeXin;Deng, LiXin;Xu, EnBu;Wang, YiZhen;Liu, Lin;Tong, Chao;Wang, XueBing;Sun, Yu;Sun, Yu;Liu, Fang;Deng, LiXin;Tong, Chao;Sun, Tao;Wang, XueBing;Tong, Chao;Sun, Tao;Sun, Tao
关键词:Endometritis; lipopolysaccharide; mRNAs; high-throughput sequencing
-
Positional cloning identified HvTUBULIN8 as the candidate gene for round lateral spikelet (RLS) in barley (Hordeum vulgare L.)
作者:Qin, Dandan;Liu, Gang;Xu, Fuchao;Xu, Qing;Peng, Yanchun;Ge, Shuangtao;Dong, Jing;Qin, Dandan;Liu, Gang;Xu, Fuchao;Xu, Qing;Peng, Yanchun;Ge, Shuangtao;Dong, Jing;Liu, Rui;Dong, Guoqing;Wang, Chunchao;Guo, Ganggang;Ling, Yu;Li, Chengdao
关键词: